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  Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors

Bäcker, M., Koch, C., Eiben, S., Geiger, F., Eber, F., Gliemann, H., et al. (2017). Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors. Sensors and Actuators b-Chemical, 238, 716-722. doi:10.1016/j.snb.2016.07.096.

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SensorsActuatorsB_238_2017_716.pdf (beliebiger Volltext), 2MB
 
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 Urheber:
Bäcker, Matthias, Autor
Koch , Claudia, Autor
Eiben , Sabine, Autor
Geiger, Fania1, Autor           
Eber , Fabian, Autor
Gliemann , Hartmut, Autor
Poghossian , Arshak, Autor
Wege , Christina, Autor
Schöning , Michael J., Autor
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Schlagwörter: Tobacco mosaic virus; Coat protein; Enzyme nanocarrier; Glucose biosensor; Virus/biomolecule nanohybrid
 Zusammenfassung: The conjunction of (bio-)chemical recognition elements with nanoscale biological building blocks such as virus particles is considered as a very promising strategy for the creation of biohybrids opening novel opportunities for label-free biosensing. This work presents a new approach for the development of biosensors using tobacco mosaic virus (TMV) nanotubes or coat proteins (CPs) as enzyme nanocarriers. Sensor chips combining an array of Pt electrodes loaded with glucose oxidase (GOD)-modified TMV nanotubes or CP aggregates were used for amperometric detection of glucose as a model system for the first time. The presence of TMV nanotubes or CPs on the sensor surface allows binding of a high amount of precisely positioned enzymes without substantial loss of their activity, and may also ensure accessibility of their active centers for analyte molecules. Specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CPs was achieved via bioaffinity binding. These layouts were tested in parallel with glucose sensors with adsorptively immobilized [SA]-GOD, as well as [SA]-GOD crosslinked with glutardialdehyde, and came out to exhibit superior sensor performance. The achieved results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for future applications in biosensorics and biochips.

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Sprache(n): eng - English
 Datum: 2016-07-092016-05-182016-07-192016-07-092017-01
 Publikationsstatus: Erschienen
 Seiten: 7
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/j.snb.2016.07.096
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Titel: Sensors and Actuators b-Chemical
  Andere : Sens. Actuator B-Chem.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Lausanne, Switzerland : Elsevier
Seiten: - Band / Heft: 238 Artikelnummer: - Start- / Endseite: 716 - 722 Identifikator: ISSN: 0925-4005
CoNE: https://pure.mpg.de/cone/journals/resource/954928549300