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  Dual functionalization of rod-shaped viruses on single coat protein subunits

Wege, C., & Geiger, F. (2018). Dual functionalization of rod-shaped viruses on single coat protein subunits. In C. Wege, & G. P. Lomonossoff (Eds.), Virus-Derived Nanoparticles for Advanced Technologies (pp. 405-424). New York, NY: Humana Press. doi:10.1007/978-1-4939-7808-3_27.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-1156-A Version Permalink: https://hdl.handle.net/21.11116/0000-0003-CFAB-4
Genre: Book Chapter
Alternative Title : Dual Functionalization of Rod-Shaped Viruses on Single Coat Protein Subunits

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MethMolBiol_1776_2018_405.pdf (Any fulltext), 593KB
 
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https://link.springer.com/content/pdf/10.1007%2F978-1-4939-7808-3_27.pdf (Any fulltext)
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 Creators:
Wege , Christina, Author
Geiger, Fania1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: Coat protein dual functionalization; Degree of labeling; Fluorescent labeling; Surface modification; Tobacco mosaic virus
 Abstract: Plant viruses are emerging as versatile tools for nanotechnology applications since it is possible to modify their multivalent protein surfaces and thereby introduce and display new functionalities. In this chapter, we describe a tobacco mosaic virus (TMV) variant that exposes two selectively addressable amino acid moieties on each of its 2130 coat protein (CP) subunits. A lysine as well as a cysteine introduced at accessible sites of every CP can be modified with amino- and/or thiol-reactive chemistry such as N-hydroxysuccinimide esters (NHS ester) and maleimide containing reagents alone or simultaneously. This enables the pairwise immobilization of distinct molecules in close vicinity to each other on the TMV surface by simple standard conjugation protocols. We describe the generation of the mutations, the virus propagation and isolation as well as the dual functionalization of the TMV variant with two fluorescent dyes. The labeling is evaluated by SDS-PAGE and spectrophotometry and the degree of labeling (DOL) calculated.

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Language(s): eng - English
 Dates: 2018-06-052018
 Publication Status: Issued
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4939-7808-3_27
URI: https://www.ncbi.nlm.nih.gov/pubmed/29869257
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Title: Virus-Derived Nanoparticles for Advanced Technologies
Source Genre: Book
 Creator(s):
Wege, Christina, Editor
Lomonossoff, George P., Editor
Affiliations:
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Publ. Info: New York, NY : Humana Press
Pages: - Volume / Issue: 1776 Sequence Number: - Start / End Page: 405 - 424 Identifier: ISBN: 978-1-4939-7806-9
ISBN: 978-1-4939-7808-3