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  Involvement of the mitogen-activated protein kinase SIMK in regulation of root hair tip growth.

Samaj, J., Ovecka, M., Hlavacka, A., Lecourieux, F., Meskiene, I., Lichtscheidl, I., et al. (2002). Involvement of the mitogen-activated protein kinase SIMK in regulation of root hair tip growth. The EMBO Journal, 21(13), 3296-3306. doi:10.1093/emboj/cdf349.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-1296-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1299-D
Genre: Journal Article

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2642144.pdf (Publisher version), 557KB
 
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Samaj, J., Author
Ovecka, M., Author
Hlavacka, A., Author
Lecourieux, F., Author
Meskiene, I., Author
Lichtscheidl, I., Author
Lenart, P.1, Author              
Salaj, J., Author
Volkmann, D., Author
Bögre, L., Author
Baluška, F., Author
Hirt, H., Author
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

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 Abstract: Mitogen-activated protein kinases (MAPKs) are involved in stress signaling to the actin cytoskeleton in yeast and animals. We have analyzed the function of the stress-activated alfalfa MAP kinase SIMK in root hairs. In epidermal cells, SIMK is predominantly nuclear. During root hair formation, SIMK was activated and redistributed from the nucleus into growing tips of root hairs possessing dense F-actin meshworks. Actin depolymerization by latrunculin B resulted in SIMK relocation to the nucleus. Conversely, upon actin stabilization with jasplakinolide, SIMK co-localized with thick actin cables in the cytoplasm. Importantly, latrunculin B and jasplakinolide were both found to activate SIMK in a root-derived cell culture. Loss of tip-focused SIMK and actin was induced by the MAPK kinase inhibitor UO 126 and resulted in aberrant root hairs. UO 126 inhibited targeted vesicle trafficking and polarized growth of root hairs. In contrast, overexpression of gain-of-function SIMK induced rapid tip growth of root hairs and could bypass growth inhibition by UO 126. These data indicate that SIMK plays a crucial role in root hair tip growth.

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Language(s): eng - English
 Dates: 2002-07-012002
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/emboj/cdf349
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Title: The EMBO Journal
Source Genre: Journal
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Pages: - Volume / Issue: 21 (13) Sequence Number: - Start / End Page: 3296 - 3306 Identifier: -