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  Fluorescent labelling of Beet necrotic yellow vein virus and Beet soil-borne mosaic virus for co- and superinfection experiments in Nicotiana benthamiana.

Laufer, M., Mohammad, H., Christ, D. S., Riedel, D., Maiss, E., Varrelmann, M., et al. (2018). Fluorescent labelling of Beet necrotic yellow vein virus and Beet soil-borne mosaic virus for co- and superinfection experiments in Nicotiana benthamiana. Journal of General Virology, 99(9), 1321-1330. doi:10.1099/jgv.0.001122.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-1312-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1314-2
Genre: Journal Article

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 Creators:
Laufer, M., Author
Mohammad, H., Author
Christ, D. S., Author
Riedel, D.1, Author              
Maiss, E., Author
Varrelmann, M., Author
Liebe, S., Author
Affiliations:
1Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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Free keywords: coinfection, BSBMV, fluorescent labelling, read-through domain, superinfection, BNYVV
 Abstract: Infectious full-length clones of Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV), both genus Benyvirus, were used for fluorescent labelling with the objective to study their interaction in coinfection and superinfection experiments. Fluorescent labelling was achieved by replacing a part of the RNA2 encoded coat protein read-through domain with either GFP or mRFP fluorescent marker proteins. This resulted in a translational fusion comprising the coat and the fluorescent protein. The labelled viruses were infectious and moved systemically in Nicotiana benthamiana, producing wild-type-like symptoms. Virus particles could be observed by electron microscopy, demonstrating that the viral read-through domain is dispensable for particle formation. Coinfection experiments revealed a spatial separation of differentially labelled populations of both identical and different Benyvirus species after N. benthamiana agro-inoculation. Identical observations were obtained when Tobacco rattle virus (TRV) was differentially labelled and used for coinfection. In contrast, coinfections of BSBMV with Potato virus X (PVX) or TRV resulted in many co-infected cells lacking spatial separation. Micro-projectile co-bombardment of N. benthamiana leaves revealed that two differently labelled populations of the same virus co-infected only a few cells before starting to separate. In superinfection experiments with N. benthamiana, BSBMV and BNYVV were unable to establish a secondary infection in plants that were previously infected with BNYVV or BSBMV. Taken together, this is the first work to describe the interaction between two economically important Benyviruses using fluorescence-labelled full-length clones.

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Language(s): eng - English
 Dates: 2018-07-302018-09-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1099/jgv.0.001122
 Degree: -

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Title: Journal of General Virology
Source Genre: Journal
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Pages: - Volume / Issue: 99 (9) Sequence Number: - Start / End Page: 1321 - 1330 Identifier: -