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  Assessing phototoxicity in live fluorescence imaging.

Laissue, P. P., Alghamdi, R. A., Tomancak, P., Reynaud, E. G., & Shroff, H. (2017). Assessing phototoxicity in live fluorescence imaging. Nature methods, 14(7), 657-661. doi:10.1038/nmeth.4344.

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Laissue, P Philippe, Author
Alghamdi, Rana A, Author
Tomancak, Pavel1, Author           
Reynaud, Emmanuel G., Author
Shroff, Hari, Author
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, ou_2340692              

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 Abstract: Are the answers to biological questions obtained via live fluorescence microscopy substantially affected by phototoxicity? Although a single set of standards for assessing phototoxicity cannot exist owing to the breadth of samples and experimental questions associated with biological imaging, we need quantitative, practical assessments and reporting standards to ensure that imaging has a minimal impact on observed biological processes and sample health. Here we discuss the problem of phototoxicity in biology and suggest guidelines to improve its reporting and assessment.

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 Dates: 2017-06-29
 Publication Status: Issued
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 Identifiers: DOI: 10.1038/nmeth.4344
Other: cbg-6887
PMID: 28661494
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Title: Nature methods
  Other : Nat Methods
Source Genre: Journal
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Pages: - Volume / Issue: 14 (7) Sequence Number: - Start / End Page: 657 - 661 Identifier: -