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  Semisynthetic sensor proteins enable metabolic assays at the point of care

Yu, Q., Xue, L., Hiblot, J., Griss, R., Fabritz, S., Roux, C., et al. (2018). Semisynthetic sensor proteins enable metabolic assays at the point of care. Science, 361(6407), 1122-1126. doi:10.1126/science.aat7992.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-1693-F Version Permalink: http://hdl.handle.net/21.11116/0000-0002-1694-E
Genre: Journal Article

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Science_361_2018_1122.pdf (Any fulltext), 706KB
 
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 Creators:
Yu, Qiuliyang1, Author              
Xue, Lin1, Author              
Hiblot, Julien1, Author              
Griss , Rudolf, Author
Fabritz, Sebastian1, Author              
Roux, Clothilde, Author
Binz, Pierre-Alain, Author
Haas, Dorothea, Author
Okun, Jürgen G., Author
Johnsson, Kai1, Author              
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1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: Monitoring metabolites at the point of care could improve the diagnosis and management of numerous diseases. Yet for most metabolites, such assays are not available. We introduce semisynthetic, light-emitting sensor proteins for use in paper-based metabolic assays. The metabolite is oxidized by nicotinamide adenine dinucleotide phosphate, and the sensor changes color in the presence of the reduced cofactor, enabling metabolite quantification with the use of a digital camera. The approach makes any metabolite that can be oxidized by the cofactor a candidate for quantitative point-of-care assays, as shown for phenylalanine, glucose, and glutamate. Phenylalanine blood levels of phenylketonuria patients were analyzed at the point of care within minutes with only 0.5 microliters of blood. Results were within 15% of those obtained with standard testing methods.

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Language(s): eng - English
 Dates: 2018-04-052018-08-092018-09-142018-09-14
 Publication Status: Published in print
 Pages: 5
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aat7992
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Title: Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 361 (6407) Sequence Number: - Start / End Page: 1122 - 1126 Identifier: ISSN: 0036-8075
CoNE: /journals/resource/991042748276600_1