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  Surface immobilization of viruses and nanoparticles elucidates early events in clathrin-mediated endocytosis

Fratini, M., Wiegand, T., Funaya, C., Jiang, Z., Pranav, S. N., Spatz, J. P., et al. (2018). Surface immobilization of viruses and nanoparticles elucidates early events in clathrin-mediated endocytosis. ACS infectious diseases, 4(11), 1585-1600. doi:10.1021/acsinfecdis.8b00134.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-19DB-C Version Permalink: http://hdl.handle.net/21.11116/0000-0002-7B71-5
Genre: Journal Article

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 Creators:
Fratini, Marta1, Author              
Wiegand, Tina1, Author              
Funaya, Charlotta, Author
Jiang, Zhongxiang, Author
Pranav, Shah N.M., Author
Spatz, Joachim P.1, Author              
Cavalcanti-Adam, Elisabetta Ada1, Author              
Boulant , Steeve, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: viruses, nanoparticles, endocytosis, clathrin-mediated endocytosis, membrane curvature, click chemistry
 Abstract: Clathrin-mediated endocytosis (CME) is an important entry pathway for viruses. Here, we applied click chemistry to covalently immobilize reovirus on surfaces to study CME during early host-pathogen interactions. To uncouple chemical and physical properties of viruses and determine their impact on CME initiation, we used the same strategy to covalently immobilize nanoparticles of different sizes. Using fluorescence live microscopy and electron microscopy, we confirmed that clathrin recruitment depends on particle size and discovered that the maturation into clathrin-coated vesicles (CCVs) is independent from cargo internalization. Surprisingly, we found that the final size of CCVs appears to be imprinted on the clathrin coat at early stages of cargo-cell interactions. Our approach has allowed us to unravel novel aspects of early interaction between viruses and clathrin machinery that influence late stages of CME and CCVs formation. This method can be easily and broadly applied to the field of nanotechnology, endocytosis and virology.

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Language(s): eng - English
 Dates: 2018-05-312018-09-102018-09-10
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: ACS infectious diseases
Source Genre: Journal
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Publ. Info: Washington, DC : ACS Publ. : American Chemical Society
Pages: - Volume / Issue: 4 (11) Sequence Number: - Start / End Page: 1585 - 1600 Identifier: ISSN: 2373-8227