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  Similar effects of electroporational stress and treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate on vimentin expression in mouse plasmacytoma cells

Rauscher, A., Giese, G., Nickel, J., & Traub, P. (2000). Similar effects of electroporational stress and treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate on vimentin expression in mouse plasmacytoma cells. Biochimica et Biophysica Acta: BBA, 1493(1-2), 170-179. doi:10.1016/S0167-4781(00)00184-6.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-45C9-E Version Permalink: http://hdl.handle.net/21.11116/0000-0002-45CC-B
Genre: Journal Article

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BBA_1493_2000_170.pdf (Any fulltext), 519KB
 
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 Creators:
Rauscher, Andreas, Author
Giese, Günter1, Author              
Nickel, Joachim, Author
Traub, Peter2, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: Vimentin; Gene expression; Electroporation; Stress; 12-O-Tetradecanoylphorbol-13-acetate; Plasmacytoma; MPC-11 cell
 Abstract: In mouse plasmacytoma cells (MPC-11), an activation of the normally repressed vimentin gene was observed as a response to transfectional stress. Effects of electroporation on vimentin gene expression were compared at the cellular and chromatin level to those caused by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). At the cellular level, similar changes in vimentin gene activity and cell-cycle distribution were observed by flow cytometry, whereas at the chromatin level similar changes in patterns of hypersensitive regions were detected by DNase I mapping. Additionally, a region located 700 bp upstream of the transcriptional start became hypersensitive to DNase I digestion upon electroporation and TPA treatment. This region overlaps two adjacent AP-1-like binding elements and generates specific DNA/AP-1 complexes in bandshift experiments. Therefore, the transcription factor AP-1 seems to play a central role in the activation of vimentin gene expression induced by these 2 different forms of stress.

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Language(s): eng - English
 Dates: 2000-07-102000-03-032000-07-182000-09-062000-09-07
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Biochimica et Biophysica Acta : BBA
  Other : Biochimica et Biophysica Acta (BBA) - Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1493 (1-2) Sequence Number: - Start / End Page: 170 - 179 Identifier: Other: 1879-2642
CoNE: https://pure.mpg.de/cone/journals/resource/18792642