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  A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons.

Kuner, T., & Augustine, G. J. (2000). A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons. Neuron, 27(3), 447-459. doi:10.1016/S0896-6273(00)00056-8.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-466F-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-4670-1
Genre: Journal Article

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Neuron_27_2000_447.pdf (Any fulltext), 268KB
 
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 Creators:
Kuner, Thomas1, Author              
Augustine, George J., Author
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1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: We constructed a novel optical indicator for chloride ions by fusing the chloride-sensitive yellow fluorescent protein with the chloride-insensitive cyan fluorescent protein. The ratio of FRET-dependent emission of these fluorophores varied in proportion to the concentration of Cl and was used to measure intracellular chloride concentration ([Cl-]i) in cultured hippocampal neurons. [Cl-]i decreased during neuronal development, consistent with the shift from excitation to inhibition during maturation of GABAergic synapses. Focal activation of GABAA receptors caused large changes in [Cl-]i that could underlie use-dependent depression of GABA-dependent synaptic transmission. GABA-induced changes in somatic [Cl-]i spread into dendrites, suggesting that [Cl-]i can signal the location of synaptic activity. This genetically encoded indicator will permit new approaches ranging from high-throughput drug screening to direct recordings of synaptic Cl- signals in vivo.

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Language(s): eng - English
 Dates: 2000-07-112000-08-302001-04-112000-09-01
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 27 (3) Sequence Number: - Start / End Page: 447 - 459 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565