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  The interaction landscape between transcription factors and the nucleosome.

Zhu, F., Farnung, L., Kaasinen, E., Sahu, B., Yin, Y., Wei, B., et al. (2018). The interaction landscape between transcription factors and the nucleosome. Nature, 562(7725), 76-81. doi:10.1038/s41586-018-0549-5.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-47E2-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-3D03-6
Genre: Journal Article

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 Creators:
Zhu, F., Author
Farnung, L.1, Author              
Kaasinen, E., Author
Sahu, B., Author
Yin, Y., Author
Wei, B., Author
Dodonova, S. O.1, Author              
Nitta, K. R., Author
Morgunova, E., Author
Taipale, M., Author
Cramer, P.1, Author              
Taipale, J., Author
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: Nucleosomes cover most of the genome and are thought to be displaced by transcription factors in regions that direct gene expression. However, the modes of interaction between transcription factors and nucleosomal DNA remain largely unknown. Here we systematically explore interactions between the nucleosome and 220 transcription factors representing diverse structural families. Consistent with earlier observations, we find that the majority of the studied transcription factors have less access to nucleosomal DNA than to free DNA. The motifs recovered from transcription factors bound to nucleosomal and free DNA are generally similar. However, steric hindrance and scaffolding by the nucleosome result in specific positioning and orientation of the motifs. Many transcription factors preferentially bind close to the end of nucleosomal DNA, or to periodic positions on the solvent-exposed side of the DNA. In addition, several transcription factors usually bind to nucleosomal DNA in a particular orientation. Some transcription factors specifically interact with DNA located at the dyad position at which only one DNA gyre is wound, whereas other transcription factors prefer sites spanning two DNA gyres and bind specifically to each of them. Our work reveals notable differences in the binding of transcription factors to free and nucleosomal DNA, and uncovers a diverse interaction landscape between transcription factors and the nucleosome.

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Language(s): eng - English
 Dates: 2018-09-242018-10-04
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41586-018-0549-5
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Title: Nature
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Pages: - Volume / Issue: 562 (7725) Sequence Number: - Start / End Page: 76 - 81 Identifier: -