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  The APT complex is involved in non-coding RNA transcription and is distinct from CPF.

Lidschreiber, M., Easter, A. D., Battaglia, S., Rodríguez-Molina, J. B., Casañal, A., Carminati, M., et al. (2018). The APT complex is involved in non-coding RNA transcription and is distinct from CPF. Nucleic Acids Research, 46(21), 11528-11538. doi:10.1093/nar/gky845.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-47EA-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-8650-B
Genre: Journal Article

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 Creators:
Lidschreiber, M.1, Author              
Easter, A. D., Author
Battaglia, S.1, Author              
Rodríguez-Molina, J. B., Author
Casañal, A., Author
Carminati, M., Author
Baejen, C.1, Author              
Grzechnik, P., Author
Maier, K. C.1, Author              
Cramer, P.1, Author              
Passmore, L. A., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: The 3'-ends of eukaryotic pre-mRNAs are processed in the nucleus by a large multiprotein complex, the cleavage and polyadenylation factor (CPF). CPF cleaves RNA, adds a poly(A) tail and signals transcription termination. CPF harbors four enzymatic activities essential for these processes, but how these are coordinated remains poorly understood. Several subunits of CPF, including two protein phosphatases, are also found in the related 'associated with Pta1' (APT) complex, but the relationship between CPF and APT is unclear. Here, we show that the APT complex is physically distinct from CPF. The 21 kDa Syc1 protein is associated only with APT, and not with CPF, and is therefore the defining subunit of APT. Using ChIP-seq, PAR-CLIP and RNA-seq, we show that Syc1/APT has distinct, but possibly overlapping, functions from those of CPF. Syc1/APT plays a more important role in sn/snoRNA production whereas CPF processes the 3'-ends of protein-coding pre-mRNAs. These results define distinct protein machineries for synthesis of mature eukaryotic protein-coding and non-coding RNAs.

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Language(s): eng - English
 Dates: 2018-09-212018-11-30
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/nar/gky845
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 46 (21) Sequence Number: - Start / End Page: 11528 - 11538 Identifier: -