English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Two-component latency distributions indicate two-step vesicular release at simple glutamatergic synapses.

Miki, T., Nakamura, Y., Malagon, G., Neher, E., & Marty, A. (2018). Two-component latency distributions indicate two-step vesicular release at simple glutamatergic synapses. Nature Communications, 9: 3943. doi:10.1038/s41467-018-06336-5.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-47FF-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-AA39-E
Genre: Journal Article

Files

show Files
hide Files
:
3001373.pdf (Publisher version), 965KB
Name:
3001373.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
3001373_Suppl_1.pdf (Supplementary material), 2MB
Name:
3001373_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3001373_Suppl_2.pdf (Supplementary material), 395KB
Name:
3001373_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Miki, T., Author
Nakamura, Y., Author
Malagon, G., Author
Neher, E.1, Author              
Marty, A., Author
Affiliations:
1Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society, ou_1571137              

Content

show
hide
Free keywords: -
 Abstract: It is often assumed that only stably docked synaptic vesicles can fuse following presynaptic action potential stimulation. However, during action potential trains docking sites are increasingly depleted, raising the question of the source of synaptic vesicles during sustained release. We have recently developed methods to reliably measure release latencies during high frequency trains at single synapses between parallel fibers and molecular layer interneurons. The latency distribution exhibits a single fast component at train onset but contains both a fast and a slow component later in the train. The contribution of the slow component increases with stimulation frequency and with release probability and decreases when blocking the docking step with latrunculin. These results suggest that the slow component reflects sequential docking and release in immediate succession. The transition from fast to slow component, as well as a later transition to asynchronous release, appear as successive adaptations of the synapse to maintain fidelity at the expense of time accuracy.

Details

show
hide
Language(s): eng - English
 Dates: 2018-09-26
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-018-06336-5
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 14 Volume / Issue: 9 Sequence Number: 3943 Start / End Page: - Identifier: -