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  Gene expression and ultrastructure of meso- and thermophilic methanotrophic consortia.

Krukenberg, V., Riedel, D., Gruber-Vodicka, H. R., Buttigieg, P. L., Tegetmeyer, H. E., Boetius, A., et al. (2018). Gene expression and ultrastructure of meso- and thermophilic methanotrophic consortia. Environmental Microbiology, 20(5), 1651-1666. doi:10.1111/1462-2920.14077.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-4E7E-B Version Permalink: http://hdl.handle.net/21.11116/0000-0003-7B06-D
Genre: Journal Article

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 Creators:
Krukenberg, V., Author
Riedel, D.1, Author              
Gruber-Vodicka, H. R., Author
Buttigieg, P. L., Author
Tegetmeyer, H. E., Author
Boetius, A., Author
Wegener, G., Author
Affiliations:
1Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Abstract: The sulfate-dependent, anaerobic oxidation of methane (AOM) is an important sink for methane in marine environments. It is carried out between anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB) living in syntrophic partnership. In this study, we compared the genomes, gene expression patterns and ultrastructures of three phylogenetically different microbial consortia found in hydrocarbon-rich environments under different temperature regimes: ANME-1a/HotSeep-1 (60 degrees C), ANME-1a/Seep-SRB2 (37 degrees C) and ANME-2c/Seep-SRB2 (20 degrees C). All three ANME encode a reverse methanogenesis pathway: ANME-2c encodes all enzymes, while ANME-1a lacks the gene for N5,N10-methylene tetrahydromethanopterin reductase (mer) and encodes a methylenetetrahydrofolate reductase (Met). The bacterial partners contain the genes encoding the canonical dissimilatory sulfate reduction pathway. During AOM, all three consortia types highly expressed genes encoding for the formation of flagella or type IV piii and/or c-type cytochromes, some predicted to be extracellular. ANME-2c expressed potentially extracellular cytochromes with up to 32 hemes, whereas ANME-1a and SRB expressed less complex cytochromes (<= 8 and <= 12 heme respectively). The intercellular space of all consortia showed nanowire-like structures and heme-rich areas. These features are proposed to enable interspecies electron exchange, hence suggesting that direct electron transfer is a common mechanism to sulfate-dependent AOM, and that both partners synthesize molecules to enable it.

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Language(s): eng - English
 Dates: 2018-02-222018-05
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1111/1462-2920.14077
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Title: Environmental Microbiology
Source Genre: Journal
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Pages: - Volume / Issue: 20 (5) Sequence Number: - Start / End Page: 1651 - 1666 Identifier: -