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  Influence of the extracellular matrix on water mobility in subcortical gray matter

Georgi, J., Metere, R., Jäger, C., Morawski, M., & Möller, H. E. (2019). Influence of the extracellular matrix on water mobility in subcortical gray matter. Magnetic Resonance in Medicine, 81(2), 1265-1279. doi:10.1002/mrm.27459.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-4EE1-9 Version Permalink: https://hdl.handle.net/21.11116/0000-0004-5025-8
Genre: Journal Article

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 Creators:
Georgi, Jakob1, Author           
Metere, Riccardo1, Author           
Jäger, Carsten2, 3, Author           
Morawski, Markus 3, Author
Möller, Harald E.1, 4, Author           
Affiliations:
1Methods and Development Unit Nuclear Magnetic Resonance, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634558              
2Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              
3Paul Flechsig Institute for Brain Research, University of Leipzig, Germany, ou_persistent22              
4Felix Bloch Institute for Solid State Physics, University of Leipzig, Germany, ou_persistent22              

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Free keywords: Diffusion; Extracellular matrix; Spin–lattice relaxation; Spin–spin relaxation; Water compartments
 Abstract: Purpose Water mobility in tissues is related to the microstructure that modulates diffusion and spin relaxation. Previous work has shown that the extracellular matrix (ECM) impacts water diffusion in cartilage. To investigate if similar contributions to image contrast exist for brain, which is characterized by a substantially lower ECM content, diffusion and relaxation were studied in fixed samples from goat and human thalamus before and after enzymatic digestion of ECM compounds. Selected experiments in human corpus callosum were included for comparing subcortical gray matter and white matter. Methods Digestion of matrix components was achieved by treatment with hyaluronidase. Nonlocalized pulsed field gradient measurements were performed with values between 0.6 and 18,000 s/mm² at 3T and temperatures between 0°C and 20°C, in addition to T1 and T2 relaxation measurements. The data were fitted to multiexponential models to account for different water compartments. After the measurements, the samples were sliced and stained for ECM‐sensitive markers to verify efficient digestion. Results Microstructural alterations associated with hyaluronan digestion did not lead to measurable effects on water diffusion or . However, T1 of the main relaxographic component, attributed to intra‐/extracellular water, decreased by 7%. Conclusion Investigations with very strong gradients did not reveal a detectable effect on water diffusion or after hyaluronan removal, indicating that the brain ECM content is too low to produce a detectable effect. The subtle alteration of T1 upon hyaluronidase treatment might reflect a modulation of intercompartmental water exchange properties.

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Language(s): eng - English
 Dates: 2018-06-122018-05-022018-06-292018-09-142019-02
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/mrm.27459
PMID: 30276849
Other: Epub 2018
 Degree: -

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Project name : -
Grant ID : HA‐314
Funding program : -
Funding organization : Helmholtz Alliance “ICEMED—Imaging and Curing Environmental Metabolic Diseases”
Project name : Ultra-High Field Magnetic Resonance Imaging / HIMR
Grant ID : 316716
Funding program : Funding Programme 7 (FP7-PEOPLE-2012-ITN)
Funding organization : European Commission (EC)
Project name : Ultrafast and temporally precise information processing: Normal and dysfunctional hearing / SPP 1608
Grant ID : MO2249/2‐1
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : Computational Connectomics / SPP 2041
Grant ID : MO2249/3‐1
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : -
Grant ID : -
Funding program : -
Funding organization : Free State of Saxony

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Title: Magnetic Resonance in Medicine
Source Genre: Journal
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Publ. Info: New York : Wiley-Liss
Pages: - Volume / Issue: 81 (2) Sequence Number: - Start / End Page: 1265 - 1279 Identifier: ISSN: 0740-3194
CoNE: https://pure.mpg.de/cone/journals/resource/954925538149