Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Crystallography on a chip – without the chip: sheet-on-sheet sandwich

Doak, B., Nass Kovács, G., Gorel, A., Foucar, L., Barends, T., Grünbein, M. L., et al. (2018). Crystallography on a chip – without the chip: sheet-on-sheet sandwich. Acta Crystallographica Section D: Structural Biology, 74, 1000-1007. doi:10.1107/S2059798318011634.

Item is

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: http://hdl.handle.net/21.11116/0000-0002-50B5-7 Versions-Permalink: http://hdl.handle.net/21.11116/0000-0002-C0D9-0
Genre: Zeitschriftenartikel
Alternativer Titel : Crystallography on a chip–without the chip: sheet-on-sheet sandwich

Dateien

einblenden: Dateien
ausblenden: Dateien
:
ActaCrystD_74_2018_1000.pdf (beliebiger Volltext), 2MB
 
Datei-Permalink:
-
Name:
ActaCrystD_74_2018_1000.pdf
Beschreibung:
-
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, Heidelberg; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://journals.iucr.org/d/issues/2018/10/00/tz5098/tz5098.pdf (beliebiger Volltext)
Beschreibung:
-
externe Referenz:
https://doi.org/10.1107/S2059798318011634 (beliebiger Volltext)
Beschreibung:
-

Urheber

einblenden:
ausblenden:
 Urheber:
Doak, Bruce1, Autor              
Nass Kovács, Gabriela1, Autor              
Gorel, Alexander1, Autor              
Foucar, Lutz1, Autor              
Barends, Thomas1, Autor              
Grünbein, Marie Luise1, Autor              
Hilpert, Mario1, Autor              
Kloos, Marco1, Autor              
Roome, Christopher M.1, Autor              
Shoeman, Robert L.1, Autor              
Stricker, Miriam1, Autor              
Tono , Kensuke, Autor
You , Daehyun, Autor
Ueda, Kiyoshi, Autor
Sherrell, Darren A., Autor
Owene, Robin L., Autor
Schlichting, Ilme1, Autor              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Inhalt

einblenden:
ausblenden:
Schlagwörter: serial crystallography; room-temperature data collection; low dose; fixed target; XFEL; Mylar sandwich chip; high throughput
 Zusammenfassung: Crystallography chips are fixed-target supports consisting of a film (for example Kapton) or wafer (for example silicon) that is processed using semiconductor-microfabrication techniques to yield an array of wells or through-holes in which single microcrystals can be lodged for raster-scan probing. Although relatively expensive to fabricate, chips offer an efficient means of high-throughput sample presentation for serial diffraction data collection at synchrotron or X-ray free-electron laser (XFEL) sources. Truly efficient loading of a chip (one microcrystal per well and no wastage during loading) is nonetheless challenging. The wells or holes must match the microcrystal size of interest, requiring that a large stock of chips be maintained. Raster scanning requires special mechanical drives to step the chip rapidly and with micrometre precision from well to well. Here, a `chip-less' adaptation is described that essentially eliminates the challenges of loading and precision scanning, albeit with increased, yet still relatively frugal, sample usage. The device consists simply of two sheets of Mylar with the crystal solution sandwiched between them. This sheet-on-sheet (SOS) sandwich structure has been employed for serial femtosecond crystallography data collection with micrometre-sized crystals at an XFEL. The approach is also well suited to time-resolved pump–probe experiments, in particular for long time delays. The SOS sandwich enables measurements under XFEL beam conditions that would damage conventional chips, as documented here. The SOS sheets hermetically seal the sample, avoiding desiccation of the sample provided that the X-ray beam does not puncture the sheets. This is the case with a synchrotron beam but not with an XFEL beam. In the latter case, desiccation, setting radially outwards from each punched hole, sets lower limits on the speed and line spacing of the raster scan. It is shown that these constraints are easily accommodated.

Details

einblenden:
ausblenden:
Sprache(n): eng - Englisch
 Datum: 2018-05-212018-08-162018
 Publikationsstatus: Im Druck veröffentlicht
 Seiten: 8
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1107/S2059798318011634
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Acta Crystallographica Section D: Structural Biology
  Kurztitel : Acta Cryst. D
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Chester, England : International Union of Crystallography
Seiten: - Band / Heft: 74 Artikelnummer: - Start- / Endseite: 1000 - 1007 Identifikator: ISSN: 2059-7983
CoNE: https://pure.mpg.de/cone/journals/resource/2059-7983