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  Crystallography on a chip – without the chip: sheet-on-sheet sandwich

Doak, B., Nass Kovács, G., Gorel, A., Foucar, L., Barends, T., Grünbein, M. L., et al. (2018). Crystallography on a chip – without the chip: sheet-on-sheet sandwich. Acta Crystallographica Section D: Structural Biology, 74, 1000-1007. doi:10.1107/S2059798318011634.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-50B5-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-C0D9-0
Genre: Journal Article
Alternative Title : Crystallography on a chip–without the chip: sheet-on-sheet sandwich

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 Creators:
Doak, Bruce1, Author              
Nass Kovács, Gabriela1, Author              
Gorel, Alexander1, Author              
Foucar, Lutz1, Author              
Barends, Thomas1, Author              
Grünbein, Marie Luise1, Author              
Hilpert, Mario1, Author              
Kloos, Marco1, Author              
Roome, Christopher M.1, Author              
Shoeman, Robert L.1, Author              
Stricker, Miriam1, Author              
Tono , Kensuke, Author
You , Daehyun, Author
Ueda, Kiyoshi, Author
Sherrell, Darren A., Author
Owene, Robin L., Author
Schlichting, Ilme1, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: serial crystallography; room-temperature data collection; low dose; fixed target; XFEL; Mylar sandwich chip; high throughput
 Abstract: Crystallography chips are fixed-target supports consisting of a film (for example Kapton) or wafer (for example silicon) that is processed using semiconductor-microfabrication techniques to yield an array of wells or through-holes in which single microcrystals can be lodged for raster-scan probing. Although relatively expensive to fabricate, chips offer an efficient means of high-throughput sample presentation for serial diffraction data collection at synchrotron or X-ray free-electron laser (XFEL) sources. Truly efficient loading of a chip (one microcrystal per well and no wastage during loading) is nonetheless challenging. The wells or holes must match the microcrystal size of interest, requiring that a large stock of chips be maintained. Raster scanning requires special mechanical drives to step the chip rapidly and with micrometre precision from well to well. Here, a `chip-less' adaptation is described that essentially eliminates the challenges of loading and precision scanning, albeit with increased, yet still relatively frugal, sample usage. The device consists simply of two sheets of Mylar with the crystal solution sandwiched between them. This sheet-on-sheet (SOS) sandwich structure has been employed for serial femtosecond crystallography data collection with micrometre-sized crystals at an XFEL. The approach is also well suited to time-resolved pump–probe experiments, in particular for long time delays. The SOS sandwich enables measurements under XFEL beam conditions that would damage conventional chips, as documented here. The SOS sheets hermetically seal the sample, avoiding desiccation of the sample provided that the X-ray beam does not puncture the sheets. This is the case with a synchrotron beam but not with an XFEL beam. In the latter case, desiccation, setting radially outwards from each punched hole, sets lower limits on the speed and line spacing of the raster scan. It is shown that these constraints are easily accommodated.

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Language(s): eng - English
 Dates: 2018-05-212018-08-162018
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S2059798318011634
 Degree: -

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Title: Acta Crystallographica Section D: Structural Biology
  Abbreviation : Acta Cryst. D
Source Genre: Journal
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Publ. Info: Chester, England : International Union of Crystallography
Pages: - Volume / Issue: 74 Sequence Number: - Start / End Page: 1000 - 1007 Identifier: ISSN: 2059-7983
CoNE: https://pure.mpg.de/cone/journals/resource/2059-7983