ausblenden:
Schlagwörter:
-
Zusammenfassung:
Phytohormones, especially jasmonates, are known to be mediators of the plant
responses to wounding and herbivore feeding. Their role in such stress responses has
been largely studied locally in treated leaves. However, less is known about the induced
systemic distribution of phytohormone signals upon these kinds of stresses. Here, a
holistic approach was performed in order to investigate the systemic phytohormone
pattern in the rosette of Arabidopsis thaliana after herbivore-related wounding. Levels of
different stress-related phytohormones such as jasmonates, abscisic acid, and salicylic
acid were analyzed in individual leaves. We demonstrate that the typically used sampling
method, where leaves are first cut and immediately frozen, causes false-positive results
since cutting already induces systemic jasmonate elevations within less than 1.6 min.
Therefore, this approach is not suitable to study systemic phytohormone changes
in the whole plant. By developing a new method where leaves are frozen first and
subsequently cut, sampling-induced phytohormone elevations could be reduced. Using
this new method, we show that jasmonic acid and its active isoleucine conjugate
(jasmonoyl-isoleucine) are involved in the fast systemic wound response of Arabidopsis.
A systemic induction of the jasmonates’ precursor, 12-oxo-phytodienoic acid, was
not observed throughout our treatments. The systemic phytohormone distribution
pattern is strongly linked to the vascular connections between the leaves, providing
further evidence that the vascular system is used for long distance-signaling in
Arabidopsis. Besides already known vascular connections, we also demonstrate that
the systemic distribution of jasmonate signals can be extended to distant leaves,
which are systemically but indirectly connected via another vascularly connected leaf.
This holistic approach covering almost the whole Arabidopsis rosette introduces a
method to overcome false-positive results in systemic phytohormone determinations
and demonstrates that wounding-induced long-distance signaling includes fast changes
in jasmonate levels in systemic, non-treated leaves.
