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Free keywords:
ACID AMIDE HYDROLASE; ENDOCANNABINOID SYSTEM; NEUROPATHIC-PAIN;
PREFRONTAL CORTEX; ABDOMINAL-PAIN; CB1; BEHAVIOR; TRPV1; MODULATION;
CHANNELSNeurosciences & Neurology; visceral pain; cannabinoid receptor type 1; brain; affective;
pancreatitis;
Abstract:
Endocannabinoids acting through cannabinoid receptor type 1 (CB1) are major modulators of peripheral somatic and visceral nociception. Although only partially studied, some evidence suggests a particular role of CB1 within the brain in nociceptive processes. As the endocannabinoid system regulates affect and emotional behaviors, we hypothesized that cerebral CB1 influences affective processing of visceral pain-related behaviors in laboratory animals. To study nocifensive responses modulated by supraspinal CB1, we used conditional knock-out mice lacking CB1 either in cortical glutamatergic neurons (Glu-CBI-KO), or in forebrain GABAergic neurons (GABA-CBI-KO), or in principal neurons of the forebrain (CaMK-CBI-KO). These mutant mice and mice treated with the CB1 antagonist SR141716 were tested for different pain-related behaviors. In an acetic acid-induced abdominal constriction test, supraspinal CB1 deletions did not affect nocifensive responses. In the cerulein-model of acute pancreatitis, mechanical allodynia or hyperalgesia were not changed, but Glu-CB1- and CaMK-CBI-KO mice showed significantly increased facial grimacing scores indicating increased affective responses to this noxious visceral stimulus. Similarly, these brain-specific CB1 KO mice also showed significantly changed thermal nociception in a hot-plate test. These results reveal a novel, and important role of CB1 expressed by cortical glutamatergic neurons in the affective component of visceral nociception. (C) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
