English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography

Salesa, A., Picart, C., & Kemkemer, R. (2019). Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography. Experimental Cell Research, 374(1), 1-11. doi:10.1016/j.yexcr.2018.10.008.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-66D9-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-02E2-8
Genre: Journal Article

Files

show Files
hide Files
:
ExpCellRes_374_2019_1.pdf (Any fulltext), 4MB
 
File Permalink:
-
Name:
ExpCellRes_374_2019_1.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ExpCellRes_374_2019_1_Suppl.pdf (Supplementary material), 887KB
 
File Permalink:
-
Name:
ExpCellRes_374_2019_1_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Salesa, Adrià, Author
Picart, Catherine, Author
Kemkemer, Ralf1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: aging; endothelial cells; microtopography; migration; protrusions
 Abstract: Cell migration is part of many important in vivo biological processes and is influenced by chemical and physical factors such as substrate topography. Although the migratory behavior of different cell types on structured substrates has already been investigated, up to date it is largely unknown if specimen's age affects cell migration on structures. In this work, we investigated age-dependent migratory behavior of human endothelial cells from young (≤ 31 years old) and old (≥ 60 years old) donors on poly(dimethylsiloxane) microstructured substrates consisting of well-defined parallel grooves. We observed a decrease in cell migration velocity in all substrate conditions and in persistence length perpendicular to the grooves in cells from old donors. Nevertheless, in comparison to young cells, old cells exhibited a higher cell directionality along grooves of certain depths and a higher persistence time. We also found a systematic decrease of donor age-dependent responses of cell protrusions in orientation, velocity and length, all of them decreased in old cells. These observations lead us to hypothesize a possible impairment of actin cytoskeleton network and affected actin polymerization and steering systems, caused by aging.

Details

show
hide
Language(s): eng - English
 Dates: 2018-10-122018-06-112018-10-152018-10-182019-01-01
 Publication Status: Published in print
 Pages: 28
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Experimental Cell Research
  Other : Exp. Cell Res.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Diego, CA : Academic Press
Pages: - Volume / Issue: 374 (1) Sequence Number: - Start / End Page: 1 - 11 Identifier: ISSN: 0014-4827
CoNE: https://pure.mpg.de/cone/journals/resource/954922645016