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  Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography

Salesa, A., Picart, C., & Kemkemer, R. (2019). Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography. Experimental Cell Research, 374(1), 1-11. doi:10.1016/j.yexcr.2018.10.008.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-66D9-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-02E2-8
Genre: Journal Article

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 Creators:
Salesa, Adrià, Author
Picart, Catherine, Author
Kemkemer, Ralf1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: aging; endothelial cells; microtopography; migration; protrusions
 Abstract: Cell migration is part of many important in vivo biological processes and is influenced by chemical and physical factors such as substrate topography. Although the migratory behavior of different cell types on structured substrates has already been investigated, up to date it is largely unknown if specimen's age affects cell migration on structures. In this work, we investigated age-dependent migratory behavior of human endothelial cells from young (≤ 31 years old) and old (≥ 60 years old) donors on poly(dimethylsiloxane) microstructured substrates consisting of well-defined parallel grooves. We observed a decrease in cell migration velocity in all substrate conditions and in persistence length perpendicular to the grooves in cells from old donors. Nevertheless, in comparison to young cells, old cells exhibited a higher cell directionality along grooves of certain depths and a higher persistence time. We also found a systematic decrease of donor age-dependent responses of cell protrusions in orientation, velocity and length, all of them decreased in old cells. These observations lead us to hypothesize a possible impairment of actin cytoskeleton network and affected actin polymerization and steering systems, caused by aging.

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Language(s): eng - English
 Dates: 2018-10-122018-06-112018-10-152018-10-182019-01-01
 Publication Status: Published in print
 Pages: 28
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.yexcr.2018.10.008
URI: https://www.ncbi.nlm.nih.gov/pubmed/30342990
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Title: Experimental Cell Research
  Other : Exp. Cell Res.
Source Genre: Journal
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Publ. Info: San Diego, CA : Academic Press
Pages: - Volume / Issue: 374 (1) Sequence Number: - Start / End Page: 1 - 11 Identifier: ISSN: 0014-4827
CoNE: https://pure.mpg.de/cone/journals/resource/954922645016