English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Methods for microbiota analysis: sample collection and laboratory methods

Ibrahim, S., & Belheouane, M. (2018). Methods for microbiota analysis: sample collection and laboratory methods. In G. Ragab, & T. P. Atkinson (Eds.), The Microbiome in Rheumatic Diseases and Infection (pp. 13-27). Cham: Springer International Publishing. doi:10.1007/978-3-319-79026-8_2.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-676F-F Version Permalink: http://hdl.handle.net/21.11116/0000-0002-6770-C
Genre: Book Chapter

Files

show Files
hide Files
:
Ibrahim-Belheouane2018_Chapter_MethodsForMicrobiotaAnalysisSa.pdf (Publisher version), 492KB
 
File Permalink:
-
Name:
Ibrahim-Belheouane2018_Chapter_MethodsForMicrobiotaAnalysisSa.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, Plön; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Ibrahim, S.1, Author
Belheouane, Meriem1, Author              
Affiliations:
1Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              

Content

show
hide
Free keywords: Study design; Laboratory methods; Combination of several techniques; Sampling strategies; Sample collection
 Abstract: The human body hosts a myriad of complex and diversified microbial assemblages on various barrier organs. The symbiotic microbial communities impact several aspects of the host’s biology in health and disease. Nowadays, microbiota research has become a central area of investigation involving diverse fields such as immunology, nutrition, and genetics. Consequently, methods for microbiota studies, comprising study design and laboratory techniques, are central in addressing general and specific research questions and especially in providing clear and reliable insights. Fortunately, approaches that investigate different aspects of microbial communities have greatly improved and expanded allowing unprecedented levels of characterization of host-microbiota interactions. Through this chapter, we aim to describe the diverse techniques available for researchers to examine bacterial communities associated with numerous human body sites. We describe various culture-based and sequencing methods, additional techniques that characterize several aspects of the entire bacterial community, as well as approaches that define the status of single cells. Moreover, we describe the advantageous strategy of combining distinct approaches in a microbiota study to collect different and complementary insights on the community and/or to confirm each different method’s outcome. Additionally, we thoroughly discuss the crucial steps of study design, sampling strategies, and sample collection and processing which influence the interpretation and conclusions of microbiota study. Overall, this chapter clarifies the critical steps and available investigation tools in a microbiota study and thus assists researchers in defining the experimental design and laboratory methods to conduct a microbiota study.

Details

show
hide
Language(s): eng - English
 Dates: 2018-06-202018
 Publication Status: Published in print
 Pages: 13-27
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1007/978-3-319-79026-8_2
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Microbiome in Rheumatic Diseases and Infection
Source Genre: Book
 Creator(s):
Ragab, Gaafar, Editor
Atkinson, T. Prescott, Editor
Matthew L., Stoll, Author
Affiliations:
-
Publ. Info: Cham : Springer International Publishing
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 13 - 27 Identifier: ISBN: 978-331979026-8
ISBN: 978-331979025-1