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  The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

Schulz, E.-C., Mehrabi, P., Müller-Werkmeister, H., Tellkamp, F., Jha, A., Stuart, W., et al. (2018). The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. Nature methods, 15(11), 901-904. doi:10.1038/s41592-018-0180-2.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-6B31-F Version Permalink: http://hdl.handle.net/21.11116/0000-0002-7FBC-D
Genre: Journal Article

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https://dx.doi.org/10.1038/s41592-018-0180-2 (Publisher version)
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 Creators:
Schulz, E.-C.1, Author              
Mehrabi, P.1, Author              
Müller-Werkmeister, H.1, 2, Author              
Tellkamp, F.3, Author              
Jha, A.1, Author              
Stuart, W.1, Author
Persch, E.4, Author
De Gasparo, R.4, Author
Diederich, F.4, Author
Pai, E. F.5, 6, 7, 8, Author
Miller, R. J. D.1, 9, 10, Author              
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Physical Chemistry, Institute of Chemistry, University of Potsdam, ou_persistent22              
3Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
4Laboratorium für Organische Chemie, ETH Zürich, ou_persistent22              
5Department of Biochemistry, University of Toronto, ou_persistent22              
6Department of Medical Biophysics, University of Toronto, ou_persistent22              
7Department of Molecular Genetics, University of Toronto, ou_persistent22              
8The Campbell Family Cancer Research Institute, Ontario Cancer Institute, Toronto, ou_persistent22              
9Departments of Chemistry and Physics, University of Toronto, ou_persistent22              
10Department of Physics, Centre for Ultrafast Imaging, University of Hamburg, ou_persistent22              

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 Abstract: We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

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Language(s): eng - English
 Dates: 2018-02-202018-08-102018-10-302018-10-30
 Publication Status: Published in print
 Pages: 4
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41592-018-0180-2
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: 4 Volume / Issue: 15 (11) Sequence Number: - Start / End Page: 901 - 904 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556