Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Collection of phloem sap in phytoplasma-infected plants

Zimmermann, M. R., Knauer, T., & Furch, A. C. U. (2018). Collection of phloem sap in phytoplasma-infected plants. In R. Musetti, & L. Pagliari (Eds.), Phytoplasmas: Methods and Protocols (pp. 291-299). New York: Springer Science+Business Media. doi:10.1007/978-1-4939-8837-2_21.

Item is

Dateien

einblenden: Dateien
ausblenden: Dateien
:
GER532.pdf (Verlagsversion), 519KB
 
Datei-Permalink:
-
Name:
GER532.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Zimmermann, Matthias R., Autor
Knauer, Torsten1, Autor           
Furch, Alexandra C. U., Autor
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Phytoplasmas colonize specifically the phloem sieve elements (SEs) of plants and influence effectively the
plant physiology. To study and understand the interaction of phytoplasmas and host plants an access to the
cellular, microscale volume of SEs is demanded. Different methods are suitable to collect phloem sap of
phytoplasma-infected plants. The two most common methods are the EDTA-facilitated exudation and the
stylectomy. For the EDTA-facilitated method, the cut end of a leaf is placed into an EDTA solution. The
EDTA prevents and avoids the Ca2+ dependent (re-) occlusion of SEs by binding Ca2+ ions and the mass
flow of SEs is restarted which results in an outflow of the SE content into the EDTA bathing solution. The
advantage is on the one hand a simple application and secondly, feasible for all plant species.
The stylectomy method requires piercing-sucking insects like any aphids. During phloem-sap ingestion,
the stylet is severed by a microcautery device or a laser from the insect body. Due to the high turgor pressure
of the SEs the phloem sap is forced out through the remaining stylet and can be collected with a glass
capillary, for example. The stylectomy delivers pure phloem sap, however, the collected volumes are in the
range of nano liters and the temporal and staff costs are tremendous. A third method is the spontaneous
exudation in phytoplasma-infected apple trees providing only in springtime large volumes of vascular sap
after cutting along the bark. For the spontaneous exudation the proportion of phloem sap is unclear. Thus,
this third method still needs a closer examination in prospective surveys.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 20182018-10-262018
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: Anderer: GER532
DOI: 10.1007/978-1-4939-8837-2_21
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Phytoplasmas: Methods and Protocols
Genre der Quelle: Buch
 Urheber:
Musetti, Rita, Herausgeber
Pagliari, Laura, Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: New York : Springer Science+Business Media
Seiten: 362 Band / Heft: - Artikelnummer: - Start- / Endseite: 291 - 299 Identifikator: ISSN: 1064-3745
ISBN: 978-1-4939-8836-5
DOI: 10.1007/978-1-4939-8837-2