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  Ab initio structure determination from experimental fluctuation X-ray scattering data

Pande, K., Donatelli, J. J., Malmerberg, E., Foucar, L., Bostedt, C., Schlichting, I., et al. (2018). Ab initio structure determination from experimental fluctuation X-ray scattering data. Proceedings of the National Academy of Sciences of the United States of America, 115(46), 11772-117777. doi:10.1073/pnas.1812064115.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-6BB8-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-D515-6
Genre: Journal Article

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 Creators:
Pande, Kanupriya, Author
Donatelli, Jeffrey J., Author
Malmerberg, Erik, Author
Foucar, Lutz1, Author              
Bostedt, Christoph, Author
Schlichting, Ilme1, Author              
Zwart, Petrus H., Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: fluctuation X-ray scattering; small-angle scattering; free-electron laser
 Abstract: Fluctuation X-ray scattering (FXS) is an emerging experimental technique in which X-ray solution scattering data are collected from particles in solution using ultrashort X-ray exposures generated by a free-electron laser (FEL). FXS experiments overcome the low data-to-parameter ratios associated with traditional solution scattering measurements by providing several orders of magnitude more information in the final processed data. Here we demonstrate the practical feasibility of FEL-based FXS on a biological multiple-particle system and describe data-processing techniques required to extract robust FXS data and significantly reduce the required number of snapshots needed by introducing an iterative noise-filtering technique. We showcase a successful ab initio electron density reconstruction from such an experiment, studying the Paramecium bursaria Chlorella virus (PBCV-1).

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Language(s): eng - English
 Dates: 2018-07-122018-10-29
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.1812064115
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 115 (46) Sequence Number: - Start / End Page: 11772 - 117777 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230