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  Zymoseptoria tritici histone modifications distinguish core and accessory chromosomes and play an important role in genome stability

Moeller, M., Schotanus, K., Soyer, J., Freitag, M., & Stukenbrock, E. H. (2018). Zymoseptoria tritici histone modifications distinguish core and accessory chromosomes and play an important role in genome stability. Phytopathology, 108(10), 282-283. doi:10.1094/PHYTO-108-10-S1.240.

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 Creators:
Moeller, M., Author
Schotanus, K., Author
Soyer, J.1, Author           
Freitag, M., Author
Stukenbrock, E. H.1, Author           
Affiliations:
1Max Planck Fellow Group Environmental Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2068284              

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 Abstract: Zymoseptoria tritici is a fungal pathogen of wheat. T he genome of the reference iso late comprises 21 chromosomes of which eight are accessory. The se chromosomes are not key determina nts for virulence, as in other fungal pathogens, however, they ar e partly conserved among dis tinct Zymoseptoria species. Accessory chromosomes are highly instable during meios is and mitosis, transcriptionally repressed and show enrichment of repetitive elements and heterochromatic histone marks . To elucidate if heterochroma tic histone modifications have an impact on chromosome stabilit y, we created deletion mutants of the methyltransferases responsible for H3K27me3 and H3K9me3. H3K27me3 is specifica lly associated with accessory chr omosomes and subtelomeric regions in Z. tritici , while H3K9me3 is associated w ith repetitive elements. We comb ined experimental evolution, genetic and high- resolution microscopic analyses t o follow the impact of these d eletions on chromosome and geno me stability. We used ChIP-seq, genome sequencing and RNA-seq to compare changes in chromatin and genome structur e and differences in gene expre ssion between mutant and wild ty pe strains. The loss of H3K9me3 results in dramatic c hromatin reorganization, transp osable element activation, genom e rearrangements, formation of “neochromosomes” and increased accessory chromos ome instability. Loss of H3K27me 3, however, has little effect on chromatin organization and tra nsposon control, but increases stability of the accessory chromosomes. We conclude t hat H3K9me3 strongly impacts ch romatin and genome organization and that H3K27me3 has an important role in stab ility of accessory chromosomes. Ep igenetic regulation is an important driver of genome evolution in this fungus and can contribute to rapid adaptation.

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Language(s): eng - English
 Dates: 2018-102018-10
 Publication Status: Published in print
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 Identifiers: DOI: 10.1094/PHYTO-108-10-S1.240
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Title: International Congress of Plant Pathology (ICPP)
Place of Event: Boston
Start-/End Date: 2018-07-29 - 2018-08-03

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Title: Phytopathology
  Other : Phytopathology
Source Genre: Journal
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Publ. Info: St. Paul, Minn., etc. : American Phytopathological Society, Supplement S1
Pages: - Volume / Issue: 108 (10) Sequence Number: - Start / End Page: 282 - 283 Identifier: ISSN: 0031-949X
CoNE: https://pure.mpg.de/cone/journals/resource/954925433418