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  Anaerobic degradation of phenanthrene by a sulfate‐reducing enrichment culture

Himmelberg, A. M., Brüls, T., Farmani, Z., Weyrauch, P., Barthel, G., Schrader, W., et al. (2018). Anaerobic degradation of phenanthrene by a sulfate‐reducing enrichment culture. Environmental Microbiology, 20(10), 3589-3600. doi:10.1111/1462-2920.14335.

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 Creators:
Himmelberg, Anna M.1, Author
Brüls, Thomas2, 3, Author
Farmani, Zahra4, 5, Author           
Weyrauch, Philip4, Author
Barthel, Gabriele1, Author
Schrader, Wolfgang5, Author           
Meckenstock, Rainer U.4, Author
Affiliations:
1Institute of Groundwater Ecology, Helmholtz Zentrum München, Neuherberg, Germany, ou_persistent22              
2CEA, DRF, Institut Jacob, Genoscope, Evry, France, ou_persistent22              
3CNRS‐UMR8030, Université Paris‐Saclay, Evry, France, ou_persistent22              
4Biofilm Centre, University of Duisburg‐Essen, Essen, Germany, ou_persistent22              
5Service Department Schrader (MS), Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445629              

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 Abstract: Anaerobic degradation processes are very important to attenuate polycyclic aromatic hydrocarbons (PAHs) in saturated, anoxic sediments. However, PAHs are poorly degradable, leading to very slow microbial growth and thus resulting in only a few cultures that have been enriched and studied so far. Here, we report on a new phenanthrene‐degrading, sulfate‐reducing enrichment culture, TRIP1. Genome‐resolved metagenomics and strain specific cell counting with FISH and flow cytometry indicated that the culture is dominated by a microorganism belonging to the Desulfobacteraceae family (60% of the community) and sharing 93% 16S rRNA sequence similarity to the naphthalene‐degrading, sulfate‐reducing strain NaphS2. The anaerobic degradation pathway was studied by metabolite analyses and revealed phenanthroic acid as the major intermediate consistent with carboxylation as the initial activation reaction. Further reduced metabolites were indicative of a stepwise reduction of the ring system. We were able to measure the presumed second enzyme reaction in the pathway, phenanthroate‐CoA ligase, in crude cell extracts. The reaction was specific for 2‐phenanthroic acid and did not transform other isomers. The present study provides first insights into the anaerobic degradation pathways of three‐ringed PAHs. The biochemical strategy follows principles known from anaerobic naphthalene degradation, including carboxylation and reduction of the aromatic ring system.

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Language(s): eng - English
 Dates: 2018-01-222018-06-212018-10-17
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1111/1462-2920.14335
 Degree: -

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Title: Environmental Microbiology
  Other : Environmental Microbiology and Environmental Microbiology Reports
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Science
Pages: - Volume / Issue: 20 (10) Sequence Number: - Start / End Page: 3589 - 3600 Identifier: ISSN: 1462-2912
CoNE: https://pure.mpg.de/cone/journals/resource/959328105031