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  Adhesion strategies of Dictyostelium discoideum - A force spectroscopy study

Kamprad, N., Witt, H., Schröder, M., Kreis, C. T., Bäumchen, O., Janshoff, A., et al. (2018). Adhesion strategies of Dictyostelium discoideum - A force spectroscopy study. Nanoscale, 10, 22504-22519. doi:10.1039/C8NR07107A.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-7058-D Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A10D-A
Genre: Journal Article

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 Creators:
Kamprad, Nadine1, Author              
Witt, Hannes2, Author              
Schröder, Marcel1, Author              
Kreis, Christian Titus3, Author              
Bäumchen, Oliver3, Author              
Janshoff, Andreas, Author
Tarantola, Marco1, Author              
Affiliations:
1Laboratory for Fluid Physics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              
2Max Planck Fellow Group Membrane-based biomimetic nano- and micro-compartments, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2586691              
3Group Dynamics of fluid and biological interfaces, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063300              

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 Abstract: Biological adhesion is essential for all motile cells and generally limits locomotion to suitably functionalized substrates displaying a compatible surface chemistry. However, organisms that face vastly varying environmental challenges require a different strategy. The model organism Dictyostelium discoideum (D.d.), a slime mould dwelling in the soil, faces the challenge of overcoming variable chemistry by employing the fundamental forces of colloid science. To understand the origin of D.d. adhesion, we realized and modified a variety of conditions for the amoeba comprising the absence and presence of the specific adhesion protein Substrate Adhesion A (sadA), glycolytic degradation, ionic strength, surface hydrophobicity and strength of van der Waals interactions by generating tailored model substrates. By employing AFM-based single cell force spectroscopy we could show that experimental force curves upon retraction exhibit two regimes. The first part up to the critical adhesion force can be described in terms of a continuum model, while the second regime of the curve beyond the critical adhesion force is governed by stochastic unbinding of individual binding partners and bond clusters. We found that D.d. relies on adhesive interactions based on EDL-DLVO (Electrical Double Layer-Derjaguin-Landau-Verwey-Overbeek) forces and contributions from the glycocalix and specialized adhesion molecules like sadA. This versatile mechanism allows the cells to adhere to a large variety of natural surfaces under various conditions.

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Language(s): eng - English
 Dates: 2018-11-022018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1039/C8NR07107A
 Degree: -

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Title: Nanoscale
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: 15 Volume / Issue: 10 Sequence Number: - Start / End Page: 22504 - 22519 Identifier: ISSN: 2040-3364
CoNE: https://pure.mpg.de/cone/journals/resource/2040-3364