High frequency neural spiking and autitory signaling by ultrafast red-shifted 
optogenetics

Mager, Thomas; Lopez de la Morena, David; Senn, Verena; Schlotte, Johannes; D'Errico, Anna; Feldbauer, Katrin; Wrobel, Christian; Jung, Sangyong; Bodensiek, Kai; Rankovic, Vladan; Browne, Lorcan; Huet, Antoine; Jüttner, Josephine; Wood, Philipp G.; Letzkus, Johannes J.; Moser, Tobias; Bamberg, Ernst

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High frequency neural spiking and autitory signaling by ultrafast red-shifted optogenetics

Mager, T., Lopez de la Morena, D., Senn, V., Schlotte, J., D'Errico, A., Feldbauer, K., et al. (2018). High frequency neural spiking and autitory signaling by ultrafast red-shifted optogenetics. Nature Communications, 9: 1750.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0002-7492-6 Version Permalink: https://hdl.handle.net/21.11116/0000-0002-7493-5

Genre: Journal Article

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Creators:
Mager, Thomas¹, Author
Lopez de la Morena, David², Author
Senn, Verena¹, Author
Schlotte, Johannes¹, Author
D'Errico, Anna¹, Author
Feldbauer, Katrin¹, Author
Wrobel, Christian², Author
Jung, Sangyong², Author
Bodensiek, Kai², Author
Rankovic, Vladan², Author
Browne, Lorcan², Author
Huet, Antoine², Author
Jüttner, Josephine³, Author
Wood, Philipp G.¹, Author
Letzkus, Johannes J.⁴, Author
Moser, Tobias², Author
Bamberg, Ernst¹, Author

Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, DE, ou_2068289
2Institute for Auditory Neuroscience and Inner Ear Lab University Medical Center, Göttingen, ou_persistent22
3Friedrich Miescher Institute for Biomedical Research, Basel, ou_persistent22
4Max Planck Institute for Brain Research, Max Planck Society, Max-von-Laue-Str. 4, 60438 Frankfurt am Main, DE, ou_2461692

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Abstract: Optogenetics revolutionizes basic research in neuroscience and cell biology and bears potential for medical applicaitons. We develop mutants leading to a unifying concept for the construction of various channelrhodopsins with fast closing kinetics. Due to different absorption maxima these channelrhodopsins allow fast neural photoactivation over the whole range of the visible spectrum. We focus our functional analysis on the fast-switching, red light-activated Chrimson variants, because red light has lower light scattering and marginal phototoxicity in tissues. We show paradigmatically for neurons of the cerebral cortex and the auditory nerve that the fast Chrimson mutants enable neural stimulation with firing frequencies of serveral hundred Hz. They drive spiking at high rates and temporal fidelity with low thresholds for stimulus intensity and duration. Optical cochlear implants resore auditory nerve activity in deaf mice. This demonstrates that the mutants facilitate neuroscience research and future medical applications such as hearing restoration.

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Dates: Date issued: 2018-05-01

Publication Status: Issued

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Rev. Type: Peer

Identifiers: PMID: 29717130
PMC: PMC5931537

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Title: Nature Communications

Abbreviation : Nat. Commun.

Source Genre: Journal

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Publ. Info: London : Nature Publishing Group

Pages: - Volume / Issue: 9 Sequence Number: 1750 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723