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  Pectin digestion in herbivorous beetles: Impact of pseudoenzymes exceeds that of their active counterparts

Kirsch, R., Kunert, G., Vogel, H., & Pauchet, Y. (2019). Pectin digestion in herbivorous beetles: Impact of pseudoenzymes exceeds that of their active counterparts. Frontiers in Physiology, 10: 685. doi:10.3389/fphys.2019.00685.

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http://dx.doi.org/10.3389/fphys.2019.00685 (Publisher version)
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 Creators:
Kirsch, Roy1, Author              
Kunert, Grit2, Author              
Vogel, Heiko1, Author              
Pauchet, Yannick1, Author              
Affiliations:
1Department of Entomology, Prof. D. G. Heckel, MPI for Chemical Ecology, Max Planck Society, ou_421895              
2Statistical Service, Dr. Grit Kunert, MPI for Chemical Ecology, Max Planck Society, ou_3171479              

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 Abstract: Many protein families harbor pseudoenzymes that have lost the catalytic function of their enzymatically active counterparts. Assigning alternative function and importance to these proteins is challenging. Because the evolution towards pseudoenzymes is driven by gene duplication, they often accumulate in multigene families. Plant cell wall-degrading enzymes (PCWDEs) are prominent examples of expanded gene families. The pectolytic glycoside hydrolase family 28 (GH28) allows herbivorous insects to break down the PCW polysaccharide pectin. GH28 in the Phytophaga clade of beetles contains many active enzymes but also many inactive counterparts. Using functional characterization, gene silencing, global transcriptome analyses and recordings of life history traits, we found that not only catalytically active but also inactive GH28 proteins are part of the same pectin-digesting pathway. The robustness and plasticity of this pathway and thus its importance for the beetle is supported by extremely high steady-state expression levels and counter-regulatory mechanisms. Unexpectedly, the impact of pseudoenzymes on the pectin-digesting pathway in Phytophaga beetles exceeds even the influence of their active counterparts, such as a lowered efficiency of food-to-energy conversion and a prolongation of the developmental period.

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 Dates: 2019-05-162019-05-29
 Publication Status: Published online
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 Identifiers: Other: HEC402
DOI: 10.3389/fphys.2019.00685
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Title: Frontiers in Physiology
  Other : Front. Physiol.
  Abbreviation : FPHYS
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Research Foundation
Pages: - Volume / Issue: 10 Sequence Number: 685 Start / End Page: - Identifier: ISSN: 1664-042X
CoNE: https://pure.mpg.de/cone/journals/resource/1664-042X