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  AtRsgA from Arabidopsis thaliana is important for maturation of the small subunit of the chloroplast ribosome

Janowski, M., Zoschke, R., Scharff, L. B., Martinez-Jaime, S., Ferrari, C., Proost, S., et al. (2018). AtRsgA from Arabidopsis thaliana is important for maturation of the small subunit of the chloroplast ribosome. The Plant Journal, 96(2), 404-420. Retrieved from https://doi.org/10.1111/tpj.14040.

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Genre: Zeitschriftenartikel
Alternativer Titel : The Plant Journal

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 Urheber:
Janowski, M.1, Autor           
Zoschke, R.1, Autor           
Scharff, Lars B.2, Autor
Martinez-Jaime, S.3, Autor           
Ferrari, C.4, Autor           
Proost, Sebastian5, Autor           
Ng Wei Xiong, Jonathan2, Autor
Omranian, N.6, Autor           
Musialak-Lange, M.5, Autor           
Nikoloski, Z.6, Autor           
Graf, A.3, Autor           
Schöttler, M. A.7, Autor           
Sampathkumar, A.8, Autor           
Vaid, N.9, Autor           
Mutwil, M.5, Autor           
Affiliations:
1Translational Regulation in Plants, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2324691              
2external, ou_persistent22              
3Plant Proteomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1950285              
4System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
5Regulatory Networks, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753332              
6Mathematical Modelling and Systems Biology - Nikoloski, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753310              
7Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753323              
8Plant Cell Biology and Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2253647              
9Molecular Mechanisms of Adaptation, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753319              

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Schlagwörter: ribosome assembly, chloroplast ribosome, assembly factor, 30S subunit, RsgA, Arabidopsis thaliana
 Zusammenfassung: Summary Plastid ribosomes are very similar in structure and function to the ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favorable under biological conditions it requires the activity of many assembly factors. Here we have characterized a homolog of bacterial RsgA in Arabidopsis thaliana and show that it can complement the bacterial homolog. Functional characterization of a strong mutant in Arabidopsis revealed that the protein is essential for plant viability, while a weak mutant produced dwarf, chlorotic plants that incorporated immature pre-16S ribosomal RNA into translating ribosomes. Physiological analysis of the mutant plants revealed smaller, but more numerous, chloroplasts in the mesophyll cells, reduction of chlorophyll a and b, depletion of proplastids from the rib meristem and decreased photosynthetic electron transport rate and efficiency. Comparative RNA sequencing and proteomic analysis of the weak mutant and wild-type plants revealed that various biotic stress-related, transcriptional regulation and post-transcriptional modification pathways were repressed in the mutant. Intriguingly, while nuclear- and chloroplast-encoded photosynthesis-related proteins were less abundant in the mutant, the corresponding transcripts were increased, suggesting an elaborate compensatory mechanism, potentially via differentially active retrograde signaling pathways. To conclude, this study reveals a chloroplast ribosome assembly factor and outlines the transcriptomic and proteomic responses of the compensatory mechanism activated during decreased chloroplast function.

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Sprache(n): eng - English
 Datum: 2018-07
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: URI: https://doi.org/10.1111/tpj.14040
 Art des Abschluß: -

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Titel: The Plant Journal
  Andere : Plant J.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Oxford : Blackwell Science
Seiten: - Band / Heft: 96 (2) Artikelnummer: - Start- / Endseite: 404 - 420 Identifikator: ISSN: 0960-7412
CoNE: https://pure.mpg.de/cone/journals/resource/954925579095_1