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  AtRsgA from Arabidopsis thaliana is important for maturation of the small subunit of the chloroplast ribosome

Janowski, M., Zoschke, R., Scharff, L. B., Martinez-Jaime, S., Ferrari, C., Proost, S., et al. (2018). AtRsgA from Arabidopsis thaliana is important for maturation of the small subunit of the chloroplast ribosome. The Plant Journal, 96(2), 404-420. Retrieved from https://doi.org/10.1111/tpj.14040.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-7649-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-764A-7
Genre: Journal Article
Alternative Title : The Plant Journal

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 Creators:
Janowski, M.1, Author              
Zoschke, R.1, Author              
Scharff, Lars B.2, Author
Martinez-Jaime, S.3, Author              
Ferrari, C.4, Author              
Proost, Sebastian5, Author              
Ng Wei Xiong, Jonathan2, Author
Omranian, N.6, Author              
Musialak-Lange, M.5, Author              
Nikoloski, Z.6, Author              
Graf, A.3, Author              
Schöttler, M. A.7, Author              
Sampathkumar, A.8, Author              
Vaid, N.9, Author              
Mutwil, M.5, Author              
Affiliations:
1Translational Regulation in Plants, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2324691              
2external, ou_persistent22              
3Plant Proteomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1950285              
4System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
5Regulatory Networks, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753332              
6Mathematical Modelling and Systems Biology - Nikoloski, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753310              
7Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753323              
8Plant Cell Biology and Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2253647              
9Molecular Mechanisms of Adaptation, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753319              

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Free keywords: ribosome assembly, chloroplast ribosome, assembly factor, 30S subunit, RsgA, Arabidopsis thaliana
 Abstract: Summary Plastid ribosomes are very similar in structure and function to the ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favorable under biological conditions it requires the activity of many assembly factors. Here we have characterized a homolog of bacterial RsgA in Arabidopsis thaliana and show that it can complement the bacterial homolog. Functional characterization of a strong mutant in Arabidopsis revealed that the protein is essential for plant viability, while a weak mutant produced dwarf, chlorotic plants that incorporated immature pre-16S ribosomal RNA into translating ribosomes. Physiological analysis of the mutant plants revealed smaller, but more numerous, chloroplasts in the mesophyll cells, reduction of chlorophyll a and b, depletion of proplastids from the rib meristem and decreased photosynthetic electron transport rate and efficiency. Comparative RNA sequencing and proteomic analysis of the weak mutant and wild-type plants revealed that various biotic stress-related, transcriptional regulation and post-transcriptional modification pathways were repressed in the mutant. Intriguingly, while nuclear- and chloroplast-encoded photosynthesis-related proteins were less abundant in the mutant, the corresponding transcripts were increased, suggesting an elaborate compensatory mechanism, potentially via differentially active retrograde signaling pathways. To conclude, this study reveals a chloroplast ribosome assembly factor and outlines the transcriptomic and proteomic responses of the compensatory mechanism activated during decreased chloroplast function.

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Language(s): eng - English
 Dates: 2018-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: URI: https://doi.org/10.1111/tpj.14040
 Degree: -

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Title: The Plant Journal
  Other : Plant J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 96 (2) Sequence Number: - Start / End Page: 404 - 420 Identifier: ISSN: 0960-7412
CoNE: https://pure.mpg.de/cone/journals/resource/954925579095_1