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  Marker-free tracking for motion artifact compensation and deformation measurements in optical mapping videos of contracting hearts

Christoph, J., & Luther, S. (2018). Marker-free tracking for motion artifact compensation and deformation measurements in optical mapping videos of contracting hearts. Frontiers in Physiology, 9: 1483. doi:10.3389/fphys.2018.01483.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-7A8A-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-A86F-4
Genre: Journal Article

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 Creators:
Christoph, Jan1, Author              
Luther, Stefan1, Author              
Affiliations:
1Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063288              

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Free keywords: fluorescence imaging; optical mapping; motion tracking; computer vision; cardiac arrhythmias, ventricular fibrillation, atrial fibrillation, heart rhythm disorders
 Abstract: Optical mapping is a high-resolution fluorescence imaging technique, which provides highly detailed visualizations of the electrophysiological wave phenomena, which trigger the beating of the heart. Recent advancements in optical mapping have demonstrated that the technique can now be performed with moving and contracting hearts and that motion and motion artifacts, once a major limitation, can now be overcome by numerically tracking and stabilizing the heart's motion. As a result, the optical measurement of electrical activity can be obtained from the moving heart surface in a co-moving frame of reference and motion artifacts can be reduced substantially. The aim of this study is to assess and validate the performance of a 2D marker-free motion tracking algorithm, which tracks motion and non-rigid deformations in video images. Because the tracking algorithm does not require markers to be attached to the tissue, it is necessary to verify that it accurately tracks the displacements of the cardiac tissue surface, which not only contracts and deforms, but also fluoresces and exhibits spatio-temporal physiology-related intensity changes. We used computer simulations to generate synthetic optical mapping videos, which show the contracting and fluorescing ventricular heart surface. The synthetic data reproduces experimental data as closely as possible and shows electrical waves propagating across the deforming tissue surface, as seen during voltage-sensitive imaging. We then tested the motion tracking and motion-stabilization algorithm on the synthetic as well as on experimental data. The motion tracking and motion-stabilization algorithm decreases motion artifacts approximately by 80% and achieves sub-pixel precision when tracking motion of 1-10 pixels (in a video image with 100 by 100 pixels), effectively inhibiting motion such that little residual motion remains after tracking and motion-stabilization. To demonstrate the performance of the algorithm, we present optical maps with a substantial reduction in motion artifacts showing action potential waves propagating across the moving and strongly deforming ventricular heart surface. The tracking algorithm reliably tracks motion if the tissue surface is illuminated homogeneously and shows sufficient contrast or texture which can be tracked or if the contrast is artificially or numerically enhanced. In this study, we also show how a reduction in dissociation-related motion artifacts can be quantified and linked to tracking precision. Our results can be used to advance optical mapping techniques, enabling them to image contracting hearts, with the ultimate goal of studying the mutual coupling of electrical and mechanical phenomena in healthy and diseased hearts.

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Language(s): eng - English
 Dates: 2018-11-02
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3389/fphys.2018.01483
 Degree: -

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Title: Frontiers in Physiology
Source Genre: Journal
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Publ. Info: -
Pages: 21 Volume / Issue: 9 Sequence Number: 1483 Start / End Page: - Identifier: -