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  Near-infrared STED nanoscopy with an engineered bacterial phytochrome.

Kamper, M., Ta, H., Jensen, N. A., Hell, S. W., & Jakobs, S. (2018). Near-infrared STED nanoscopy with an engineered bacterial phytochrome. Nature Communications, 9: 4762. doi:10.1038/s41467-018-07246-2.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-7E20-D Version Permalink: http://hdl.handle.net/21.11116/0000-0003-7001-D
Genre: Journal Article

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 Creators:
Kamper, M.1, Author              
Ta, H.1, Author              
Jensen, N. A.1, Author              
Hell, S. W.1, Author              
Jakobs, S.2, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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 Abstract: The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.

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Language(s): eng - English
 Dates: 2018-11-12
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-018-07246-2
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Title: Nature Communications
Source Genre: Journal
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Pages: 7 Volume / Issue: 9 Sequence Number: 4762 Start / End Page: - Identifier: -