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  Longitudinal 19F magnetic resonance imaging of brain oxygenation in a mouse model of vascular cognitive impairment using a cryogenic radiofrequency coil

Khalil, A., Mueller, S., Foddis, M., Mosch, L., Lips, J., Przesdzing, I., Temme, S., Flögel, U., Dirnagl, U., & Boehm-Sturm, P. (2019). Longitudinal 19F magnetic resonance imaging of brain oxygenation in a mouse model of vascular cognitive impairment using a cryogenic radiofrequency coil. Magnetic Resonance Materials in Physics, Biology and Medicine, 32(1), 105-144. doi:10.1007/s10334-018-0712-x.

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基本情報

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0002-87F0-6 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000D-0599-1
資料種別: 学術論文

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URL:
https://zenodo.org/record/3952438#.ZD_PwLLP2Bw (プレプリント)
説明:
-
OA-Status:
Green

作成者

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 作成者:
Khalil, Ahmed1, 2, 3, 著者           
Mueller, Susanne 1, 4, 著者
Foddis, Marco 1, 著者
Mosch, Larissa 1, 著者
Lips, Janet 1, 著者
Przesdzing, Ingo 1, 著者
Temme, Sebastian 5, 著者
Flögel, Ulrich 5, 著者
Dirnagl, Ulrich 1, 4, 6, 著者
Boehm-Sturm, Philipp 1, 4, 著者
所属:
1Center for Stroke Research, Charité University Medicine Berlin, Germany, ou_persistent22              
2Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, Leipzig, DE, ou_634549              
3Berlin School of Mind and Brain, Humboldt University Berlin, Germany, ou_persistent22              
4NeuroCure Cluster of Excellence, Charité University Medicine Berlin, Germany, ou_persistent22              
5Department of Molecular Cardiology, University Hospital Düsseldorf, Germany, ou_persistent22              
6Berlin Institute of Health (BIH), Germany, ou_persistent22              

内容説明

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キーワード: Oxygenation; Vascular cognitive impairment; 19F-MRI; Perfluoro-15-crown-5-ether
 要旨: INTRODUCTION:
We explored the use of a perfluoro-15-crown-5 ether nanoemulsion (PFC) for measuring tissue oxygenation using a mouse model of vascular cognitive impairment.
METHODS:
Seventeen C57BL/6 mice underwent stereotactic injection of PFC coupled to a fluorophore into the striatum and corpus callosum. Combined 1H/19F magnetic resonance imaging (MRI) to localize the PFC and R1 mapping to assess pO2 were performed. The effect of gas challenges on measured R1 was investigated. All mice then underwent bilateral implantation of microcoils around the common carotid arteries to induce global cerebral hypoperfusion. 19F-MRI and R1 mapping were performed 1 day, 1 week, and 4 weeks after microcoil implantation. In vivo R1 values were converted to pO2 through in vitro calibration. Tissue reaction to the PFC was assessed through ex vivo immunohistochemistry of microglial infiltration.
RESULTS:
R1 increased with increasing oxygen concentrations both in vitro and in vivo and the strength of the 19F signal remained largely stable over 4 weeks. In the two mice that received all four scans, tissue pO2 decreased after microcoil implantation and recovered 4 weeks later. We observed infiltration of the PFC deposits by microglia.
DISCUSSION:
Despite remaining technical challenges, intracerebrally injected PFC is suitable for monitoring brain oxygenation in vivo

資料詳細

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言語: eng - English
 日付: 2018-10-142018-06-292018-10-222018-11-122019-02
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: 査読あり
 識別子（DOI, ISBNなど）: DOI: 10.1007/s10334-018-0712-x
PMID: 30421249
その他: Epub ahead of print
 学位: -

関連イベント

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訴訟

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Project information

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Project name : -
Grant ID : BIH_PRO_317
Funding program : -
Funding organization : Stiftung Charité
Project name : NeuroCure - neue Perspektiven in der Therapie neurologischer Erkrankungen / EXC 257
Grant ID : -
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : Master switches bei kardialer Ischämie / SFB 1116
Grant ID : -
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : -
Grant ID : FL 303/6-1/
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : -
Grant ID : 01EO0801
Funding program : -
Funding organization : German Federal Ministry of Education and Research (BMBF)
Project name : -
Grant ID : TE 1209/1-1
Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft (DFG)

出版物 1

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出版物名: Magnetic Resonance Materials in Physics, Biology and Medicine
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: Amsterdam : No longer published by Elsevier
ページ: - 巻号: 32 (1) 通巻号: - 開始・終了ページ: 105 - 144 識別子（ISBN, ISSN, DOIなど）: ISSN: 0968-5243
CoNE: https://pure.mpg.de/cone/journals/resource/954926245532