English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Highly efficient protein-free fusion: a giant vesicle study

Lira, R. d., Robinson, T., Dimova, R., & Riske, K. A. (2019). Highly efficient protein-free fusion: a giant vesicle study. Biophysical Journal, 116(1), 79-91. doi:10.1016/j.bpj.2018.11.3128.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-98D6-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-DD07-B
Genre: Journal Article

Files

show Files
hide Files
:
Supporting Material.docx (Supplementary material), 3MB
 
File Permalink:
-
Name:
Supporting Material.docx
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
30718_2_new_final_file_264261_pyv2qp.avi (Supplementary material), 2MB
 
File Permalink:
-
Name:
30718_2_new_final_file_264261_pyv2qp.avi
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
30718_2_new_final_file_264260_phv2qp.avi (Supplementary material), 2MB
 
File Permalink:
-
Name:
30718_2_new_final_file_264260_phv2qp.avi
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
30718_2_new_final_file_264259_ptv2qp.avi (Supplementary material), 562KB
 
File Permalink:
-
Name:
30718_2_new_final_file_264259_ptv2qp.avi
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
30718_2_new_final_file_264255_p6v2q6.avi (Supplementary material), 5MB
 
File Permalink:
-
Name:
30718_2_new_final_file_264255_p6v2q6.avi
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
Article.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
Article.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
Cover.pdf (Publisher version), 262KB
 
File Permalink:
-
Name:
Cover.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
Accepted Manuscript.pdf (Any fulltext), 2MB
Name:
Accepted Manuscript.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Lira, Rafael de1, Author              
Robinson, Tom2, Author              
Dimova, Rumiana1, Author              
Riske , Karin A., Author
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              
2Tom Robinson, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288691              

Content

show
hide
Free keywords: giant vesicles, fusion intermediate, confocal microscopy, vesicle electrodeformation, FRET, FLIM-FRET, microfluidics, POPG, DOTAP
 Abstract: Membrane fusion is a ubiquitous process in biology and is a prerequisite for many intracellular delivery protocols relying on the use of liposomes as drug carriers. Here, we investigate in detail the process of membrane fusion and the role of opposite charges in a protein-free lipid system based on cationic liposomes (LUVs) and anionic giant unilamellar vesicles (GUVs) composed of different palmitoyloleoylphosphatidylcholine:palmitoyloleoylphosphatidylglycerol (POPC:POPG) molar ratios. By using a set of optical microscopy- and microfluidics-based methods, we show that liposomes strongly dock to GUVs of pure POPC or low POPG fraction (up to 10 mol%), in a process mainly associated with hemifusion and membrane tension increase, commonly leading to GUV rupture. On the other hand, docked LUVs quickly and very efficiently fuse with negative GUVs of POPG fractions at or above 20 mol%, resulting in dramatic GUV area increase in a charged-dependent manner. Importantly, both hemifusion and full fusion are leakage-free. Fusion efficiency is quantified by the lipid transfer from liposomes to GUVs upon fusion using fluorescence resonance energy transfer (FRET), which leads to con-sistent results when compared to fluorescence lifetime-based FRET. We develop an approach to deduce the final composition of single GUVs after fusion based on the FRET efficiency. We can conclude that fusion is driven by membrane charge and appears to proceed up to charge-neutralization of the acceptor GUV.

Details

show
hide
Language(s): eng - English
 Dates: 2018-112018-12-012019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1016/j.bpj.2018.11.3128
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 116 (1) Sequence Number: - Start / End Page: 79 - 91 Identifier: ISSN: 0006-3495