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  Switchable Release of Bone Morphogenetic Protein from Thermoresponsive Poly(NIPAM-co-DMAEMA)/Cellulose Sulfate Particle Coatings

Müller, M., Urban, B., Reis, B., Yu, X., Grab, A. L., Cavalcanti-Adam, E. A., et al. (2018). Switchable Release of Bone Morphogenetic Protein from Thermoresponsive Poly(NIPAM-co-DMAEMA)/Cellulose Sulfate Particle Coatings. Polymers, 10(12), 1-19. doi:10.3390/polym10121314.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-970D-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-970E-5
Genre: Journal Article

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 Creators:
Müller, Martin, Author
Urban, Birgit, Author
Reis, Berthold, Author
Yu, Xiaoqian, Author
Grab, Anna Luise, Author
Cavalcanti-Adam, Elisabetta Ada1, 2, Author              
Kuckling, Dirk, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: bone healing; protein delivery; polyelectrolyte complex; thermoresponsive polymers; bone morphogenetic protein 2
 Abstract: Thermoresponsive coatings of poly(N-isopropylacrylamide-co-DMAEMA)/cellulose sulfate (PNIPAM-DMAEMA/CS) complexes are reported eluting bone-morphogenetic-protein-2 (BMP-2) on demand relevant for implant assisted local bone healing. PNIPAM-DMAEMA/CS dispersions contained colloid particles with hydrodynamic radii RH = 170–288 nm at T = 25 °C shrinking to RH = 74–103 nm at T = 60 °C. Obviously, PNIPAM-DMAEMA/CS undergoes volume phase transition (VPT) analogously to pure PNIPAM, when critical VPT temperature (VPTT) is exceeded. Temperature dependent turbidity measurements revealed broad VPT and VPTT 47 °C for PNIPAM-DMAEMA/CS colloid dispersions at pH = 7.0. FTIR spectroscopy on thermoresponsive PNIPAM-DMAEMA/CS particle coatings at germanium model substrates under HEPES buffer indicated both wet-adhesiveness and VPT behavior based on diagnostic band intensity increases with temperature. From respective temperature courses empirical VPTT ≈ 42 °C for PNIPAM-DMAEMA/CS coatings at pH = 7.0 were found, which were comparable to VPTT found for respective dispersions. Finally, the PNIPAM-DMAEMA/CS coatings were loaded with BMP-2 and model protein papain (PAP). Time dependent FTIR spectroscopic measurements showed, that for T = 37 °C there was a relative protein release of ≈30% for PAP and ≈10% for BMP-2 after 24 h, which did not increase further. Heating to T = 42 °C for PAP and to 47 °C for BMP-2 further secondary protein release of ≈20% after 24 h was found, respectively, interesting for clinical applications. BMP-2 eluted even at 47 °C was found to be still biologically active.

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Language(s): eng - English
 Dates: 2018-11-202018-10-262018-11-222018-11-272018-11-27
 Publication Status: Published in print
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3390/polym10121314
 Degree: -

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Title: Polymers
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 10 (12) Sequence Number: - Start / End Page: 1 - 19 Identifier: ISSN: 2073-4360
CoNE: https://pure.mpg.de/cone/journals/resource/2073-4360