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  Effect of antibiotic treatment and gamma-irradiation on cuticular hydrocarbon profiles and mate choice in tsetse flies (Glossina m. morsitans)

Engl, T., Michalkova, V., Weiss, B. L., Uzel, G. D., Takac, P., Miller, W. J., et al. (2018). Effect of antibiotic treatment and gamma-irradiation on cuticular hydrocarbon profiles and mate choice in tsetse flies (Glossina m. morsitans). BMC Microbiology, 18(Suppl 1): 145. doi:10.1186/s12866-018-1292-7.

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http://dx.doi.org/10.1186/s12866-018-1292-7 (Publisher version)
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 Creators:
Engl, Tobias1, Author              
Michalkova, Veronika, Author
Weiss, Brian L., Author
Uzel, Güler D., Author
Takac, Peter, Author
Miller, Wolfgang J., Author
Abd-Alla, Adly M. M., Author
Aksoy, Serap, Author
Kaltenpoth, Martin1, Author              
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1Max Planck Research Group Insect Symbiosis, MPI for Chemical Ecology, Max Planck Society, ou_421897              

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 Abstract: Background: Symbiotic microbes represent a driving force of evolutionary innovation by conferring novel ecological traits to their hosts. Many insects are associated with microbial symbionts that contribute to their host’s nutrition, digestion, detoxification, reproduction, immune homeostasis, and defense. In addition, recent studies suggest a microbial involvement in chemical communication and mating behavior, which can ultimately impact reproductive isolation and, hence, speciation. Here we investigated whether a disruption of the microbiota through antibiotic treatment or irradiation affects cuticular hydrocarbon profiles, and possibly mate choice behavior in the tsetse fly, Glossina morsitans morsitans. Four independent experiments that differentially knock down the multiple bacterial symbionts of tsetse flies were conducted by subjecting tsetse flies to ampicillin, tetracycline, or gammairradiation and analyzing their cuticular hydrocarbon profiles in comparison to untreated controls by gas chromatography – mass spectrometry. In two of the antibiotic experiments, flies were mass-reared, while individual rearing was done for the third experiment to avoid possible chemical cross-contamination between individual flies. Results: All three antibiotic experiments yielded significant effects of antibiotic treatment (particularly tetracycline) on cuticular hydrocarbon profiles in both female and male G. m. morsitans, while irradiation itself had no effect on the CHC profiles. Importantly, tetracycline treatment reduced relative amounts of 15,19,23-trimethylheptatriacontane, a known compound of the female contact sex pheromone, in two of the three experiments, suggesting a possible implication of microbiota disturbance on mate choice decisions. Concordantly, both female and male flies preferred non-treated over tetracycline-treated flies in direct choice assays. Conclusions: While we cannot exclude the possibility that antibiotic treatment had a directly detrimental effect on fly vigor as we are unable to recolonize antibiotic treated flies with individual symbiont taxa, our results are consistent with an effect of the microbiota, particularly the obligate nutritional endosymbiont Wigglesworthia, on CHC profiles and mate choice behavior. These findings highlight the importance of considering host-microbiota interactions when studying chemical communication and mate choice in insects.

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 Dates: 20182018-11-23
 Publication Status: Published online
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 Identifiers: Other: KAL081
DOI: 10.1186/s12866-018-1292-7
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Title: BMC Microbiology
Source Genre: Journal
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Publ. Info: BioMed Central
Pages: - Volume / Issue: 18(Suppl 1) Sequence Number: 145 Start / End Page: - Identifier: ISSN: 1471-2180
CoNE: https://pure.mpg.de/cone/journals/resource/111000136905014