English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Class II cytochrome P450 reductase governs the biosynthesis of alkaloids

Parage, C., Foureau, E., Kellner, F., Burlat, V., Mahroug, S., Lanoue, A., et al. (2016). Class II cytochrome P450 reductase governs the biosynthesis of alkaloids. Plant Physiology, 172(3), 1563-1577. doi:10.1104/pp.16.00801.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
SOC063.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
SOC063.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
SOC063s1.zip (Supplementary material), 4MB
 
File Permalink:
-
Name:
SOC063s1.zip
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Type / Checksum:
application/zip
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://www.ncbi.nlm.nih.gov/pubmed/27688619 (Publisher version)
Description:
OA
OA-Status:

Creators

show
hide
 Creators:
Parage, Claire1, Author
Foureau, Emilien1, Author
Kellner, Franziska1, Author
Burlat, Vincent1, Author
Mahroug, Samira1, Author
Lanoue, Arnaud1, Author
Duge de Bernonville, Thomas1, Author
Arias Londono, Monica1, Author
Carqueijeiro, Ines1, Author
Oudin, Audrey1, Author
Besseau, Sebastien1, Author
Papon, Nicolas1, Author
Glevarec, Gaelle1, Author
Atehortua, Lucia1, Author
Giglioli-Guivarc'h, Nathalie1, Author
St-Pierre, Benoit1, Author
Clastre, Marc1, Author
O'Connor, Sarah E.1, Author           
Courdavault, Vincent1, Author
Affiliations:
1external, ou_persistent22              

Content

show
hide
Free keywords: CATHARANTHUS-ROSEUS; FUNCTIONAL EXPRESSION; P450 OXIDOREDUCTASES; SUBCELLULAR-LOCALIZATION; IRIDOID BIOSYNTHESIS; SECOLOGANIN SYNTHASE; YEAST EXPRESSION; INDOLE ALKALOIDS; MULTIPLE FORMS; NADPHPlant Sciences;
 Abstract: Expansion of the biosynthesis of plant specialized metabolites notably results from the massive recruitment of cytochrome P450s that catalyze multiple types of conversion of biosynthetic intermediates. For catalysis, P450s require a two-electron transfer catalyzed by shared cytochrome P450 oxidoreductases (CPRs), making these auxiliary proteins an essential component of specialized metabolism. CPR isoforms usually group into two distinct classes with different proposed roles, namely involvement in primary and basal specialized metabolisms for class I and inducible specialized metabolism for class II. By studying the role of CPRs in the biosynthesis of monoterpene indole alkaloids, we provide compelling evidence of an operational specialization of CPR isoforms in Catharanthus roseus (Madagascar periwinkle). Global analyses of gene expression correlation combined with transcript localization in specific leaf tissues and gene-silencing experiments of both classes of CPR all point to the strict requirement of class II CPRs for monoterpene indole alkaloid biosynthesis with a minimal or null role of class I. Direct assays of interaction and reduction of P450s in vitro, however, showed that both classes of CPR performed equally well. Such high specialization of class II CPRs in planta highlights the evolutionary strategy that ensures an efficient reduction of P450s in specialized metabolism.

Details

show
hide
Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: SOC063
DOI: 10.1104/pp.16.00801
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 172 (3) Sequence Number: - Start / End Page: 1563 - 1577 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438