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  Identification and characterization of the iridoid synthase involved in oleuropein biosynthesis in olive (Olea europaea) fruits

Alagna, F., Geu-Flores, F., Kries, H., Panara, F., Baldoni, L., O'Connor, S. E., et al. (2016). Identification and characterization of the iridoid synthase involved in oleuropein biosynthesis in olive (Olea europaea) fruits. The Journal of Biological Chemistry, 291(11), 5542-5554. doi:10.1074/jbc.M115.701276.

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SOC060.pdf (Publisher version), 4MB
 
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https://doi.org/10.1074/jbc.M115.701276 (Publisher version)
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 Creators:
Alagna, Fiammetta1, Author
Geu-Flores, Fernando1, Author
Kries, Hajo1, Author
Panara, Francesco1, Author
Baldoni, Luciana1, Author
O'Connor, Sarah E.1, Author           
Osbourn, Anne1, Author
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1external, ou_persistent22              

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Free keywords: TRANSCRIPTOME COEXPRESSION ANALYSIS; PROGESTERONE 5-BETA-REDUCTASE; PHENOLIC-COMPOUNDS; SECOIRIDOID GLUCOSIDES; 7-DEOXYLOGANETIC ACID; GENE-EXPRESSION; OIL; DEFENSE; SYRINGA; LEAVESBiochemistry & Molecular Biology; biosynthesis; enzyme; plant; secondary metabolism; transcriptomics; Oleaceae; monoterpenes; phenolic compounds; secoiridoids;
 Abstract: The secoiridoids are the main class of specialized metabolites present in olive (Olea europaea L.) fruit. In particular, the secoiridoid oleuropein strongly influences olive oil quality because of its bitterness, which is a desirable trait. In addition, oleuropein possesses a wide range of pharmacological properties, including antioxidant, anti-inflammatory, and anti-cancer activities. In accordance, obtaining high oleuropein varieties is a main goal of molecular breeding programs. Here we use a transcriptomic approach to identify candidate genes belonging to the secoiridoid pathway in olive. From these candidates, we have functionally characterized the olive homologue of iridoid synthase (OeISY), an unusual terpene cyclase that couples an NAD (P)H-dependent 1,4-reduction step with a subsequent cyclization, and we provide evidence that OeISY likely generates the monoterpene scaffold of oleuropein in olive fruits. OeISY, the first pathway gene characterized for this type of secoiridoid, is a potential target for breeding programs in a high value secoiridoid-accumulating species.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: SOC060
DOI: 10.1074/jbc.M115.701276
 Degree: -

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Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 291 (11) Sequence Number: - Start / End Page: 5542 - 5554 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1