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  De novo production of the plant-derived alkaloid strictosidine in yeast

Brown, S., Clastre, M., Courdavault, V., & O'Connor, S. E. (2015). De novo production of the plant-derived alkaloid strictosidine in yeast. Proceedings of the National Academy of Sciences of the United States of America, 112(11), 3205-3210. doi:10.1073/pnas.1423555112.

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http://dx.doi.org/10.1073/pnas.1423555112 (Verlagsversion)
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 Urheber:
Brown, Stephanie1, Autor
Clastre, Marc1, Autor
Courdavault, Vincent1, Autor
O'Connor, Sarah E.1, Autor           
Affiliations:
1external, ou_persistent22              

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Schlagwörter: GERANYL DIPHOSPHATE SYNTHASE; SACCHAROMYCES-CEREVISIAE; IRIDOID BIOSYNTHESIS; CATHARANTHUS-ROSEUS; INDOLE ALKALOIDS; BENZYLISOQUINOLINE ALKALOIDS; ESCHERICHIA-COLI; NATURAL-PRODUCTS; PATHWAY; EXPRESSIONScience & Technology - Other Topics; monoterpene indole alkaloid; strictosidine; secologanin; Catharanthus roseus; Saccharomyces cerevisiae;
 Zusammenfassung: The monoterpene indole alkaloids are a large group of plant-derived specialized metabolites, many of which have valuable pharmaceutical or biological activity. There are similar to 3,000 monoterpene indole alkaloids produced by thousands of plant species in numerous families. The diverse chemical structures found in this metabolite class originate from strictosidine, which is the last common biosynthetic intermediate for all monoterpene indole alkaloid enzymatic pathways. Reconstitution of biosynthetic pathways in a heterologous host is a promising strategy for rapid and inexpensive production of complex molecules that are found in plants. Here, we demonstrate how strictosidine can be produced de novo in a Saccharomyces cerevisiae host from 14 known monoterpene indole alkaloid pathway genes, along with an additional seven genes and three gene deletions that enhance secondary metabolism. This system provides an important resource for developing the production of more complex plant-derived alkaloids, engineering of nonnatural derivatives, identification of bottlenecks in monoterpene indole alkaloid biosynthesis, and discovery of new pathway genes in a convenient yeast host.

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Sprache(n): eng - English
 Datum: 2015
 Publikationsstatus: Erschienen
 Seiten: 6
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: Anderer: SOC051
DOI: 10.1073/pnas.1423555112
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Titel: Proceedings of the National Academy of Sciences of the United States of America
  Andere : Proc. Acad. Sci. USA
  Kurztitel : PNAS
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, D.C. : National Academy of Sciences
Seiten: - Band / Heft: 112 (11) Artikelnummer: - Start- / Endseite: 3205 - 3210 Identifikator: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230