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  Spore-autonomous fluorescent protein expression identifies meiotic chromosome mis-segregation as the principal cause of hybrid sterility in yeast.

Rogers, D. W., McConnell, E., Ono, J., & Greig, D. (2018). Spore-autonomous fluorescent protein expression identifies meiotic chromosome mis-segregation as the principal cause of hybrid sterility in yeast. PLoS Biology, 16(11): e2005066. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=132958900&site=ehost-live&scope=site.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-A2A0-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A2A1-0
Genre: Journal Article

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 Creators:
Rogers, David W.1, 2, Author              
McConnell, Ellen1, 2, Author              
Ono, Jasmine3, Author
Greig, Duncan1, 4, Author              
Affiliations:
1Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445640              
2Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2421699              
3external, ou_persistent22              
4External, ou_persistent22              

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Free keywords: FLUORESCENT proteins, CHROMOSOME segregation, SACCHAROMYCES, MEIOSIS, FUNGAL reproduction, Animal studies, Autosomes, Biochemistry, Biology and life sciences, Cell biology, Cell cycle and cell division, Cell processes, Chromosome biology, Chromosome pairs, Chromosomes, DNA, DNA recombination, Eukaryota, Experimental organism systems, Fungal reproduction, Fungal spores, Fungi, Gene expression and vector techniques, Genetics, Homologous chromosomes, Homologous recombination, Meiosis, Model organisms, Molecular biology, Molecular biology assays and analysis techniques, Molecular biology techniques, Mycology, Nucleic acids, Organisms, Protein expression, Research and analysis methods, Research Article, Saccharomyces, Saccharomyces cerevisiae, Yeast, Yeast and fungal models
 Abstract: Genome-wide sequence divergence between populations can cause hybrid sterility through the action of the anti-recombination system, which rejects crossover repair of double strand breaks between nonidentical sequences. Because crossovers are necessary to ensure proper segregation of homologous chromosomes during meiosis, the reduced recombination rate in hybrids can result in high levels of nondisjunction and therefore low gamete viability. Hybrid sterility in interspecific crosses of Saccharomyces yeasts is known to be associated with such segregation errors, but estimates of the importance of nondisjunction to postzygotic reproductive isolation have been hampered by difficulties in accurately measuring nondisjunction frequencies. Here, we use spore-autonomous fluorescent protein expression to quantify nondisjunction in both interspecific and intraspecific yeast hybrids. We show that segregation is near random in interspecific hybrids. The observed rates of nondisjunction can explain most of the sterility observed in interspecific hybrids through the failure of gametes to inherit at least one copy of each chromosome. Partially impairing the anti-recombination system by preventing expression of the RecQ helicase SGS1 during meiosis cuts nondisjunction frequencies in half. We further show that chromosome loss through nondisjunction can explain nearly all of the sterility observed in hybrids formed between two populations of a single species. The rate of meiotic nondisjunction of each homologous pair was negatively correlated with chromosome size in these intraspecific hybrids. Our results demonstrate that sequence divergence is not only associated with the sterility of hybrids formed between distantly related species but may also be a direct cause of reproductive isolation in incipient species.

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Language(s): eng - English
 Dates: 2018-11-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
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Title: PLoS Biology
  Other : PLoS Biol.
Source Genre: Journal
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Publ. Info: San Francisco, California, US : Public Library of Science
Pages: - Volume / Issue: 16 (11) Sequence Number: e2005066 Start / End Page: - Identifier: ISSN: 1544-9173
CoNE: https://pure.mpg.de/cone/journals/resource/111056649444170