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  Combined use of unnatural amino acids enables dual color super-resolution Imaging of proteins via click chemistry.

Saal, K. A., Richter, F., Rehling, P., & Rizzoli, S. O. (2018). Combined use of unnatural amino acids enables dual color super-resolution Imaging of proteins via click chemistry. ACS Nano, 12(12), 12247-12254. doi:10.1021/acsnano.8b06047.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-AF7F-C Version Permalink: http://hdl.handle.net/21.11116/0000-0002-B7FD-3
Genre: Journal Article

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 Creators:
Saal, K. A., Author
Richter, F., Author
Rehling, P.1, Author              
Rizzoli, S. O., Author
Affiliations:
1Max Planck Fellow Peter Rehling, ou_1298545              

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 Abstract: Recent advances in optical nanoscopy have brought the imaging resolution to the size of the individual macromolecules, thereby setting stringent requirements for the fluorescent labels. Such requirements are optimally fulfilled by the incorporation of unnatural amino acids (UAAs) in the proteins of interest (POI), followed by fluorophore conjugation via click chemistry. However, this approach has been limited to single POIs in mammalian cells. Here we solve this problem by incorporating different UAAs in different POIs, which are expressed in independent cell sets. The cells are then fused, thereby combining the different proteins and organelles, and are easily imaged by dual-color super-resolution microscopy. This procedure, which we termed Fuse2Click, is simple, requires only the well-established Amber codon, and allows the use of all previously optimized UAAs and tRNA/RS pairs. This should render it a tool of choice for multi-color click-based imaging.

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Language(s): eng - English
 Dates: 2018-12-102018
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acsnano.8b06047
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Title: ACS Nano
Source Genre: Journal
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Pages: - Volume / Issue: 12 (12) Sequence Number: - Start / End Page: 12247 - 12254 Identifier: -