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  The Na+/H+ exchanger Nhe1 modulates network excitability via GABA release.

Bocker, H. T., Heinrich, T., Liebmann, L., Hennings, J. C., Seemann, E., Gerth, M., et al. (2018). The Na+/H+ exchanger Nhe1 modulates network excitability via GABA release. Cerebral Cortex, (in press). doi:10.1093/cercor/bhy308.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-AF9A-C Version Permalink: http://hdl.handle.net/21.11116/0000-0003-36D8-D
Genre: Journal Article

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 Creators:
Bocker, H. T., Author
Heinrich, T., Author
Liebmann, L., Author
Hennings, J. C., Author
Seemann, E., Author
Gerth, M., Author
Jakovčevski, I., Author
Preobraschenski, J.1, Author              
Kessels, M. M., Author
Westermann, M., Author
Isbrandt, D., Author
Jahn, R.1, Author              
Qualmann, B., Author
Hübner, C. A., Author
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              

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Free keywords: ataxia, epilepsy, ion homeostasis, Lichtenstein–Knorr syndrome, synaptic pH regulation
 Abstract: Brain functions are extremely sensitive to pH changes because of the pH-dependence of proteins involved in neuronal excitability and synaptic transmission. Here, we show that the Na+/H+ exchanger Nhe1, which uses the Na+ gradient to extrude H+, is expressed at both inhibitory and excitatory presynapses. We disrupted Nhe1 specifically in mice either in Emx1-positive glutamatergic neurons or in parvalbumin-positive cells, mainly GABAergic interneurons. While Nhe1 disruption in excitatory neurons had no effect on overall network excitability, mice with disruption of Nhe1 in parvalbumin-positive neurons displayed epileptic activity. From our electrophysiological analyses in the CA1 of the hippocampus, we conclude that the disruption in parvalbumin-positive neurons impairs the release of GABA-loaded vesicles, but increases the size of GABA quanta. The latter is most likely an indirect pH-dependent effect, as Nhe1 was not expressed in purified synaptic vesicles itself. Conclusively, our data provide first evidence that Nhe1 affects network excitability via modulation of inhibitory interneurons.

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Language(s): eng - English
 Dates: 2018-12-12
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/cercor/bhy308
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Title: Cerebral Cortex
Source Genre: Journal
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Pages: 14 Volume / Issue: - Sequence Number: (in press) Start / End Page: - Identifier: -