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  Superresolution fluorescence imaging of mutant Huntingtin aggregation in cells.

Sahl, S. J., & Vonk, W. I. M. (2019). Superresolution fluorescence imaging of mutant Huntingtin aggregation in cells. In C. M. Gomes (Ed.), Protein Misfolding Diseases. New York: Humana Press. doi:10.1007/978-1-4939-8820-4_15.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-B386-C Version Permalink: http://hdl.handle.net/21.11116/0000-0002-B389-9
Genre: Book Chapter

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3016256.pdf (Publisher version), 415KB
 
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 Creators:
Sahl, S. J.1, Author              
Vonk, W. I. M., Author
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1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: Amyloid; Cellular superresolution imaging; Fibrillar aggregates; Fibrils; Fluorescence nanoscopy; Huntington’s disease; Inclusion bodies; Oligomers; Polyglutamine expansion; Trinucleotide repeat disorders
 Abstract: Fluorescence-based nanoscopy methods (also known as "superresolution" microscopy) have substantially expanded our options to examine the distributions of molecules inside cells with nanometer-scale resolution and molecular specificity. In the biophysical analysis of aggregation-prone misfolded proteins and peptides, this has enabled the visualization of distinct populations of aggregated species such as fibrillar assemblies within intact neuronal cells, well below previous limits of sensitivity and resolution. With the Huntington's disease protein, polyglutamine-expanded mutant huntingtin, as an example, we provide sample preparation and imaging protocols for superresolution microscopy down to the ~30 nm-level.

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Language(s): eng - English
 Dates: 2018-10-202019
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/978-1-4939-8820-4_15
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Title: Protein Misfolding Diseases
Source Genre: Book
 Creator(s):
Gomes, C. M., Editor
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Publ. Info: New York : Humana Press
Pages: 338 Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISBN: 978-1-4939-8819-8

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 1873 Sequence Number: - Start / End Page: 241 - 251 Identifier: -