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  High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics

Mager, T., Lopez de la Morena, D., Senn, V., Schlotte, J., D´Errico, A., Feldbauer, K., et al. (2018). High frequency neural spiking and auditory signaling by ultrafast red-shifted optogenetics. Nature Communications, 9(1): 1750. doi:10.1038/s41467-018-04146-3.

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2018
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Copyright © The Author(s) 2018

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 Creators:
Mager, Thomas, Author
Lopez de la Morena, David, Author
Senn, Verena1, Author
Schlotte, Johannes, Author
D´Errico, Anna, Author
Feldbauer, Katrin, Author
Wrobel, Christian, Author
Jung, Sangyong, Author
Bodensiek, Kai, Author
Rankovic, Vladan, Author
Browne, Lorcan, Author
Huet, Antoine, Author
Juttner, Josephine, Author
Wood, Phillip G., Author
Letzkus, Johannes J., Author
Moser, Tobias, Author
Bamberg, Ernst, Author
Affiliations:
1Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Max Planck Society, Deutschordenstr. 46, 60528 Frankfurt, DE, ou_2074314              

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Free keywords: *Action Potentials Animals Auditory Pathways/*physiology Calcium/metabolism Cell Line, Tumor Cells, Cultured Hearing/physiology Humans Mice Mutation Neurons/*physiology Optogenetics/*methods Patch-Clamp Techniques Permeability Rats Rats, Sprague-Dawley Signal Transduction Xenopus laevis
 Abstract: Optogenetics revolutionizes basic research in neuroscience and cell biology and bears potential for medical applications. We develop mutants leading to a unifying concept for the construction of various channelrhodopsins with fast closing kinetics. Due to different absorption maxima these channelrhodopsins allow fast neural photoactivation over the whole range of the visible spectrum. We focus our functional analysis on the fast-switching, red light-activated Chrimson variants, because red light has lower light scattering and marginal phototoxicity in tissues. We show paradigmatically for neurons of the cerebral cortex and the auditory nerve that the fast Chrimson mutants enable neural stimulation with firing frequencies of several hundred Hz. They drive spiking at high rates and temporal fidelity with low thresholds for stimulus intensity and duration. Optical cochlear implants restore auditory nerve activity in deaf mice. This demonstrates that the mutants facilitate neuroscience research and future medical applications such as hearing restoration.

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 Dates: 2018-05-01
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-018-04146-3
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 9 (1) Sequence Number: 1750 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723