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  The efficacy of whole human genome capture on ancient dental calculus and dentin

Ziesemer, K. A., Ramos-Madrigal, J., Mann, A. E., Brandt, B. W., Sankaranarayanan, K., Ozga, A. T., et al. (2018). The efficacy of whole human genome capture on ancient dental calculus and dentin. American Journal of Physical Anthropology, 168(3), 496-509. doi:10.1002/ajpa.23763.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-B749-E Version Permalink: http://hdl.handle.net/21.11116/0000-0003-9578-E
Genre: Journal Article

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 Creators:
Ziesemer, Kirsten A., Author
Ramos-Madrigal, Jazmín, Author
Mann, Allison E., Author
Brandt, Bernd W., Author
Sankaranarayanan, Krithivasan, Author
Ozga, Andrew T., Author
Hoogland, Menno, Author
Hofman, Courtney A., Author
Salazar-García, Domingo C.1, Author              
Frohlich, Bruno, Author
Milner, George R., Author
Stone, Anne C., Author
Aldenderfer, Mark, Author
Jr, Lewis, Author
M., Cecil, Author
Hofman, Corinne L., Author
Warinner, Christina G.1, 2, Author              
Schroeder, Hannes, Author
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              
2Kostbare Kulturen, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2591692              

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Free keywords: ancient DNA, genomics, hybridization capture, target enrichment
 Abstract: Abstract Objectives Dental calculus is among the richest known sources of ancient DNA in the archaeological record. Although most DNA within calculus is microbial, it has been shown to contain sufficient human DNA for the targeted retrieval of whole mitochondrial genomes. Here, we explore whether calculus is also a viable substrate for whole human genome recovery using targeted enrichment techniques. Materials and methods Total DNA extracted from 24 paired archaeological human dentin and calculus samples was subjected to whole human genome enrichment using in-solution hybridization capture and high-throughput sequencing. Results Total DNA from calculus exceeded that of dentin in all cases, and although the proportion of human DNA was generally lower in calculus, the absolute human DNA content of calculus and dentin was not significantly different. Whole genome enrichment resulted in up to four-fold enrichment of the human endogenous DNA content for both dentin and dental calculus libraries, albeit with some loss in complexity. Recovering more on-target reads for the same sequencing effort generally improved the quality of downstream analyses, such as sex and ancestry estimation. For nonhuman DNA, comparison of phylum-level microbial community structure revealed few differences between precapture and postcapture libraries, indicating that off-target sequences in human genome-enriched calculus libraries may still be useful for oral microbiome reconstruction. Discussion While ancient human dental calculus does contain endogenous human DNA sequences, their relative proportion is low when compared with other skeletal tissues. Whole genome enrichment can help increase the proportion of recovered human reads, but in this instance enrichment efficiency was relatively low when compared with other forms of capture. We conclude that further optimization is necessary before the method can be routinely applied to archaeological samples.

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Language(s): eng - English
 Dates: 2018-12-26
 Publication Status: Published online
 Pages: 14
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/ajpa.23763
Other: shh1146
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Title: American Journal of Physical Anthropology
Source Genre: Journal
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Publ. Info: New York, NY : Wiley-Liss, Inc.
Pages: - Volume / Issue: 168 (3) Sequence Number: - Start / End Page: 496 - 509 Identifier: ISSN: 0002-9483
CoNE: https://pure.mpg.de/cone/journals/resource/954926960915