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  Plk1/Polo phosphorylates Sas-4 at the onset of mitosis for an efficient recruitment of pericentriolar material to centrosomes.

Ramani, A., Mariappan, A., Gottardo, M., Mandad, S., Urlaub, H., Avidor-Reiss, T., et al. (2018). Plk1/Polo phosphorylates Sas-4 at the onset of mitosis for an efficient recruitment of pericentriolar material to centrosomes. Cell Reports, 25(13), 3618-3630. doi:10.1016/j.celrep.2018.11.102.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-BA03-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-AF8A-D
Genre: Journal Article

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 Creators:
Ramani, A., Author
Mariappan, A., Author
Gottardo, M., Author
Mandad, S.1, Author              
Urlaub, H.1, Author              
Avidor-Reiss, T., Author
Riparbelli, M., Author
Giuliano, C., Author
Debec, A., Author
Feederle, R., Author
Gopalakrishnan, J., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: Drosophila melanogaster; Plk1; Sas-4; centrosome maturation; centrosomes; pericentriolar material
 Abstract: Centrosomes are the major microtubule-organizing centers, consisting of centrioles surrounded by a pericentriolar material (PCM). Centrosomal PCM is spatiotemporally regulated to be minimal during interphase and expands as cells enter mitosis. It is unclear how PCM expansion is initiated at the onset of mitosis. Here, we identify that, in Drosophila, Plk1/Polo kinase phosphorylates the conserved centrosomal protein Sas-4 in vitro. This phosphorylation appears to occur at the onset of mitosis, enabling Sas-4's localization to expand outward from meiotic and mitotic centrosomes. The Plk1/Polo kinase site of Sas-4 is then required for an efficient recruitment of Cnn and γ-tubulin, bona fide PCM proteins that are essential for PCM expansion and centrosome maturation. Point mutations at Plk1/Polo sites of Sas-4 affect neither centrosome structure nor centriole duplication but specifically reduce the affinity to bind Cnn and γ-tubulin. These observations identify Plk1/Polo kinase regulation of Sas-4 as essential for efficient PCM expansion.

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Language(s): eng - English
 Dates: 2018-12-26
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.celrep.2018.11.102
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Title: Cell Reports
Source Genre: Journal
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Pages: - Volume / Issue: 25 (13) Sequence Number: - Start / End Page: 3618 - 3630 Identifier: -