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  A suite of complementary biocontrol traits allows a native consortium of root‐associated bacteria to protect their host plant from a fungal sudden‐wilt disease

Santhanam, R., Menezes, R. C., Grabe, V., Li, D., Baldwin, I. T., & Groten, K. (2019). A suite of complementary biocontrol traits allows a native consortium of root‐associated bacteria to protect their host plant from a fungal sudden‐wilt disease. Molecular Ecology, 28(5), 1154-1169. doi:10.1111/mec.15012.

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 Creators:
Santhanam, Rakesh1, 2, Author              
Menezes, Riya Christina3, Author              
Grabe, Veit4, Author              
Li, Dapeng1, 2, Author              
Baldwin, Ian Thomas1, Author              
Groten, Karin5, Author              
Affiliations:
1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              
4Microscopy Service, Dr. Veit Grabe, MPI for Chemical Ecology, Max Planck Society, ou_3171478              
5MPI for Chemical Ecology, Max Planck Society, ou_24027              

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 Abstract: The beneficial effects of plant‐bacterial interactions in controlling plant pests have been extensively studied with single bacterial isolates. However, in nature, bacteria interact with plants in multi‐taxa consortia, systems which remain poorly understood. Previously, we demonstrated that a consortium of five native bacterial isolates protected their host plant Nicotiana attenuata from a sudden wilt disease (Santhanam et al., 2015b). Here we explore the mechanisms behind the protection effect against the native pathosystem. Three members of the consortium, A70 Pseudomonas azotoformans, A176 P. frederiksbergensis, E46 Arthrobacter nitroguajacolicus form biofilms when grown individually in vitro, and the amount of biofilm increased synergistically in the 5‐membered consortium, including two Bacillus species, B. megaterium and B. mojavensis. FISH (fluorescence in‐situ hybridization) and SEM (scanning electron microscopy) in planta imaging techniques confirmed biofilm formation and revealed locally distinct distributions of the five bacterial strains colonizing different areas on the plant‐root surface. One of the five isolates, K1 B. mojavensis produces the antifungal compound, surfactin, under in vitro and in vivo conditions, clearly inhibiting fungal growth. Furthermore, isolates A70 and A176 produce siderophores under in vitro conditions. Based on these results we infer that the consortium of five bacterial isolates protects its host against fungal phytopathogens via complementary traits. The study should encourage researchers to create synthetic communities from native strains of different genera to improve bioprotection against wilting diseases.

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 Dates: 2019-01-082019-022019-03
 Publication Status: Published in print
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 Identifiers: Other: ITB596
DOI: 10.1111/mec.15012
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Title: Molecular Ecology
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 28 (5) Sequence Number: - Start / End Page: 1154 - 1169 Identifier: ISSN: 0962-1083
CoNE: https://pure.mpg.de/cone/journals/resource/954925580119