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  Bacterially Derived Antibody Binders as Small Adapters for DNA‐PAINT Microscopy

Schlichthaerle, T., Ganji, M., Auer, A., Wade, O. K., & Jungmann, R. (2019). Bacterially Derived Antibody Binders as Small Adapters for DNA‐PAINT Microscopy. ChemBioChem: A European Journal of Chemical Biology, 20(8), 1032-1038. doi:10.1002/cbic.201800743.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0003-E68E-A Version Permalink: https://hdl.handle.net/21.11116/0000-0007-8DCC-5
Genre: Journal Article
Other : Bacterial-derived antibody binders as small adapters for DNA-PAINT microscopy.
Subtitle : Communication

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https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201800743 (Publisher version)
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 Creators:
Schlichthaerle, Thomas1, Author           
Ganji, Mahipal1, Author           
Auer, Alexander1, Author           
Wade, Orsolya Kimbu1, Author           
Jungmann, Ralf1, Author           
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: DNA Nanotechnology; DNA-PAINT; Protein binders; Single-molecule microscopy; super-resolution microscopy
 Abstract: Current optical super-resolution implementations are capable of resolving features spaced just a few nanometers apart. However, translating this spatial resolution to cellular targets is limited by the large size of traditionally employed primary and secondary antibody reagents. Recent advancements in small and efficient protein binders for super-resolution microscopy such as nanobodies or aptamers provide an exciting avenue for the future, however their widespread availability is still limited. To address this issue, we here report the combination of bacterial-derived binders commonly used in antibody purification with DNA-PAINT microscopy. The small size of these protein binders compared to secondary antibodies make them an attractive labeling alternative for emerging super-resolution techniques. We here present a labeling protocol for DNA conjugation of bacterial-derived protein A and G for DNA-PAINT imaging and assay their performance intracellularly by targeting primary antibodies against Tubulin, TOM20, and EGFR and quantify the increase in obtainable resolution. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Language(s): eng - English
 Dates: 2018-12-272019-04
 Publication Status: Issued
 Pages: -
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 Table of Contents: We also acknowledge the support of the imaging facility at the MPI of Biochemistry.
 Rev. Type: -
 Identifiers: ISI: 30589198
DOI: 10.1002/cbic.201800743
 Degree: -

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Project name : DFG JU 2957/1-1, SFB1032
Grant ID : -
Funding program : Emmy Noether Program
Funding organization : Deutsche Forschungsgemeinschaft
Project name : ERC Starting Grant (MolMap)
Grant ID : 680241
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : Marie Skłodowska-Curie Actions
Grant ID : 796606
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: ChemBioChem: A European Journal of Chemical Biology
  Other : ChemBioChem
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 20 (8) Sequence Number: - Start / End Page: 1032 - 1038 Identifier: ISSN: 1439-4227
CoNE: https://pure.mpg.de/cone/journals/resource/110978984568897_1