English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Proteomics Analysis of Thermoplasma Quinone Droplets.

Nagy, I., Sun, N., Varga, S., Boicu, M., Zinzula, L., & Kukolya, J. (2019). Proteomics Analysis of Thermoplasma Quinone Droplets. Proteomics, 19(3): 1800317. doi:10.1002/pmic.201800317.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-CE16-E Version Permalink: http://hdl.handle.net/21.11116/0000-0004-0350-E
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Nagy, Istvan1, Author              
Sun, Na1, Author              
Varga, Sandor2, Author
Boicu, Marius1, Author              
Zinzula, Luca1, Author              
Kukolya, Jozsef2, Author
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

Content

show
hide
Free keywords: 1D PAGE; LC-MS/MS; Thermoplasma Quinone droplets; Thermoplasma acidophilum
 Abstract: A novel type of lipid droplet/lipoprotein (LD/LP) particle from Thermoplasma acidophilum has been identified recently, and based on biochemical evidences, it was named Thermoplasma Quinone Droplet (TaQD). The major components of TaQDs are menaquinones, and to some extent polar lipids, and the 153 amino acid long Ta0547 vitellogenin-N domain protein. In this paper, the aim is to identify TaQD proteome components with 1D-SDS-PAGE/LC-MS/MS and cross reference them with Edman degradation. TaQD samples isolated with three different purification methods-column chromatography, immunoprecipitation, and LD ultracentrifugation-are analyzed. Proteins Ta0093, Ta0182, Ta0337, Ta0437, Ta0438, Ta0547, and Ta1223a are identified as constituents of the TaQD proteome. The majority of these proteins is uncharacterized and has low molecular weight, and none of them is predicted to take part in lipid metabolism. Bioinformatics analyses does not predict any interaction between these proteins, however, there are indications of interactions with proteins taking part in lipid metabolism. Whether if TaQDs provide platform for lipid metabolism and the interactions between TaQD proteins and lipid metabolism proteins occur in the reality remain for further studies. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Details

show
hide
Language(s): eng - English
 Dates: 2018-122019-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 30520262
DOI: 10.1002/pmic.201800317
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proteomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : WILEY-VCH
Pages: - Volume / Issue: 19 (3) Sequence Number: 1800317 Start / End Page: - Identifier: ISSN: 1615-9853
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000294310