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  Dynamic Organellar Maps for Spatial Proteomics.

Itzhak, D. N., Schessner, J. P., & Borner, G. H. H. (2018). Dynamic Organellar Maps for Spatial Proteomics. Current protocols in cell biology, e81. doi:10.1002/cpcb.81.

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Genre: Journal Article

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 Creators:
Itzhak, Daniel N.1, Author              
Schessner, Julia P.1, Author              
Borner, Georg H. H.1, Author              
Affiliations:
1Borner, Georg / Systems Biology of Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_3060205              

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Free keywords: mass spectrometry; organelle proteomics; protein dynamics; spatial proteomics; subcellular localization; systems biology
 Abstract: Eukaryotic cells are highly compartmentalized and protein subcellular localization critically influences protein function. Identification of the subcellular localizations of proteins and their translocation events upon perturbation has mostly been confined to targeted studies or laborious microscopy-based methods. Here we describe a systematic mass spectrometry-based method for spatial proteomics. The approach uses simple fractionation profiling and has two applications: Firstly it can be used to infer subcellular protein localization on a proteome-wide scale, resulting in a protein map of the cell. Secondly, the method permits identification of changes in protein localization, by comparing maps made under different conditions, as a tool for unbiased systems cell biology. © 2018 by John Wiley & Sons, Inc. © 2018 John Wiley & Sons, Inc.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 30489039
DOI: 10.1002/cpcb.81
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Project name : “ToPAG—Toxic protein aggregation in neurodegeneration”
Grant ID : 318987
Funding program : ERC‐2012‐SyG_318987‐ToPAG
Funding organization : European Research Council

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Title: Current protocols in cell biology
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: e81 Start / End Page: - Identifier: ISSN: 1934-2616