English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Carbohydrate-based nanomaterials for imaging and drug delivery

Varela-Aramburu, S. (2018). Carbohydrate-based nanomaterials for imaging and drug delivery. PhD Thesis, Freie Universität, Berlin.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0002-D016-A Version Permalink: http://hdl.handle.net/21.11116/0000-0006-65AB-8
Genre: Thesis

Files

show Files
hide Files
:
Dissertation.pdf (Any fulltext), 13MB
 
File Permalink:
-
Name:
Dissertation.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute of Colloids and Interfaces, MTKG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Varela-Aramburu, Silvia1, 2, Author              
Affiliations:
1Peter H. Seeberger - Nanoparticles and Colloidal Polymers, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863307              
2Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, Potsdam-Golm Science Park, Am Mühlenberg 1 OT Golm, 14476 Potsdam, DE, ou_1863306              

Content

show
hide
Free keywords: -
 Abstract: As glycans are exposed on the surface of living cells, they are instrumental in biological processes such as cell-cell interactions, cell growth and cell differentiation. Multivalent carbohydrate-based nanomaterials help clarify these processes by mimicking the biological activity of carbohydrates. Moreover, nanomaterials have high potential for the imaging of diseases or biological processes, and can be further engineered for controlled and targeted drug delivery. In the first part of this dissertation, a straightforward and robust room temperature one-pot synthesis of ultrasmall gold nanoparticles (2 nm) was developed using thio-glucose as a reducing and stabilizing agent (Chapter 2). The resultant monodisperse gold nanoparticles showed high stability and could be further functionalized using two different conjugation methods. These non-cytotoxic nanoclusters were radiolabeled for biodistribution studies in vivo, showing accumulation in almost all organs and clearance after 24 h. The developed ultrasmall glycosylated gold nanoparticles were utilized to target the protozoan parasites Plasmodium falciparum and Toxoplasma gondii (Chapter 3). These parasites contain cysteine-rich domains in their surface proteins and could potentially capture gold nanoparticles through the well-known thiol-gold affinity. The gold nanoparticles were able to efficiently bind all the blood stages of P. falciparum, and both intracellular and extracellular T. gondii. Drug conjugation was performed for further in vitro and in vivo inhibition studies. Flat discoidal mesoporous silica nanoparticles were synthesized and their biological applications were investigated (Chapter 4). These novel materials were internalized by HeLa cancer cells and showed increased intracellular drug delivery as compared to spherical analogues. Moreover, the introduction of cleavable disulfide moieties within the mesoporous silica framework enabled investigation of the impact of increasing cleavable bonds on the breakability and efficacy of these materials as nanovectors. Multivalent glycosylated nanoparticles were synthesized to image biological processes (Chapter 5). Ultrasmall fluorescent silicon nanoparticles were functionalized with glucose XIII and a radiotracer in order to study the blood brain barrier crossing in vivo. Also, plasmonic gold nanoparticles were conjugated with a collection of carbohydrates for carbohydrateprotein interactions studies between green algae and cyanobacteria. In conclusion, the design and engineering of glycosylated nanoparticles allows mimicking biological processes, obtaining unknown information through imaging techniques, and producing targeted nanocarriers for controlled drug delivery.

Details

show
hide
Language(s):
 Dates: 20182018
 Publication Status: Published in print
 Pages: 161
 Publishing info: Berlin : Freie Universität
 Table of Contents: -
 Rev. Method: -
 Identifiers: URN: urn:nbn:de:kobv:188-refubium-22666-7
 Degree: PhD

Event

show

Legal Case

show

Project information

show

Source

show